Protein Info for Pf1N1B4_3621 in Pseudomonas fluorescens FW300-N1B4

Annotation: Flagellar biosynthesis protein FlhB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 379 transmembrane" amino acids 34 to 55 (22 residues), see Phobius details amino acids 89 to 120 (32 residues), see Phobius details amino acids 144 to 165 (22 residues), see Phobius details amino acids 191 to 215 (25 residues), see Phobius details amino acids 337 to 348 (12 residues), see Phobius details PF01312: Bac_export_2" amino acids 8 to 348 (341 residues), 426.7 bits, see alignment E=3.5e-132 TIGR00328: flagellar biosynthetic protein FlhB" amino acids 8 to 354 (347 residues), 405.9 bits, see alignment E=7.5e-126

Best Hits

KEGG orthology group: K02401, flagellar biosynthetic protein FlhB (inferred from 87% identity to pfo:Pfl01_1552)

Predicted SEED Role

"Flagellar biosynthesis protein FlhB" in subsystem Flagellar motility or Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162APU3 at UniProt or InterPro

Protein Sequence (379 amino acids)

>Pf1N1B4_3621 Flagellar biosynthesis protein FlhB (Pseudomonas fluorescens FW300-N1B4)
MAESESGQDKTEDPTEKRKKDSREKGEIARSKELNTLAVMLAGAGALLIFGGALAQDMME
LMRMNFTLSREVILDQRNMGTFLLHSGQIALLAIQPVMITLLLAALIGPISLGGWLFAAG
SMAPKFSRMNPGAGLKRMFSAKALVELLKALAKFFIVLFVALAVLSSDIDDLLRIAHEPL
DMAIIHSLQVVGWSTLWMACGLIIIAVVDVPVQLWESHKKLQMTKQEVRDEHKDQEGKPE
VKQRIRQLQREMSQRRMMAAIPEADVVITNPTHYAVALKYDPDKGNAPVLLAKGSDFLAL
KIREIAVANNVLLLESPALARSIYYSTELEQEIPGGLYLAVAQVLAYVYQIRQYRAGKGK
RPDPLQDDLPIPPDLRRDS