Protein Info for Pf1N1B4_3612 in Pseudomonas fluorescens FW300-N1B4

Annotation: Flagellar hook-length control protein FliK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 453 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF02120: Flg_hook" amino acids 318 to 398 (81 residues), 71.6 bits, see alignment E=1.9e-24

Best Hits

KEGG orthology group: K02414, flagellar hook-length control protein FliK (inferred from 76% identity to pfo:Pfl01_1544)

Predicted SEED Role

"Flagellar hook-length control protein FliK" in subsystem Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166PF10 at UniProt or InterPro

Protein Sequence (453 amino acids)

>Pf1N1B4_3612 Flagellar hook-length control protein FliK (Pseudomonas fluorescens FW300-N1B4)
MPVTPNILLQAATTAKAQAASANAPALTAEPGDKASSFAQVYANQAQNKPSAMADGSAKP
ARDKVSDSVGKKDVSNDKSAAPEPTVADSGKSLPADKPAQADGKTACDDASDTAEASTPV
ADTAPVDPALDPALVVQPVLPAPVPETPTVVATAQSQVEAPVAVVVTPAAAQTVATIDPD
FDPEADPLDALPAVRMAMEQGGHVSPASQAQPKAAPISAQVDGEPTSAQNFATGMAGMLD
VQADQDSTSQGGEKAFSGLIDDGLKDLKSASSDTRVDDFANRLAALTQAATPKTANALPV
NQPIAMHQSGWTEEVVNRVMYLSSANLKAADIQLQPAELGRLDIRLNMVPDQQTQVTFMS
AHPSVREALDGQMHRLRDMFAQQGMGQVDVNVSDQSRGWQGQGQEQAQQGQGGRANANGG
RLDSADEELPATVAEVAANTTSVIGTSAVDYYA