Protein Info for Pf1N1B4_3601 in Pseudomonas fluorescens FW300-N1B4

Annotation: Flagellar regulatory protein FleQ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 280 PF00072: Response_reg" amino acids 8 to 117 (110 residues), 95.9 bits, see alignment E=2.5e-31 PF00158: Sigma54_activat" amino acids 138 to 241 (104 residues), 146.2 bits, see alignment E=1.1e-46 PF14532: Sigma54_activ_2" amino acids 144 to 240 (97 residues), 41.9 bits, see alignment E=1.8e-14

Best Hits

Predicted SEED Role

"Flagellar regulatory protein FleQ" in subsystem Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (280 amino acids)

>Pf1N1B4_3601 Flagellar regulatory protein FleQ (Pseudomonas fluorescens FW300-N1B4)
VKDMAIKVLLVEDDRALREALADTLLLGGHDYTAVGSAEEALAAVGAEAFNLVLSDVNMP
GMDGHQLLGLLRARQPQLPVLLMTAHGAVERAVDAMRQGAADYLVKPFEPKALLDLVARH
ALGNLGATESEGPVAFEPASAQLLELAARVARSDSTVLISGESGTGKEVLARYIHQHSHR
ASQPFIAINCAAIPDNMLEATLFGHEKGSFTGAIAAQAGKFEQADGGTILLDEISEMPSA
FKPNYCACCKSAKSSESAHASRSCWTFEWWLPPTVTWPPK