Protein Info for Pf1N1B4_3578 in Pseudomonas fluorescens FW300-N1B4

Annotation: Similar to CDP-glucose 4,6-dehydratase (EC 4.2.1.45)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 360 TIGR02622: CDP-glucose 4,6-dehydratase" amino acids 10 to 356 (347 residues), 477.5 bits, see alignment E=1.3e-147 PF01370: Epimerase" amino acids 16 to 241 (226 residues), 102.9 bits, see alignment E=2.9e-33 PF02719: Polysacc_synt_2" amino acids 17 to 203 (187 residues), 34.6 bits, see alignment E=1.9e-12 PF16363: GDP_Man_Dehyd" amino acids 17 to 331 (315 residues), 107.5 bits, see alignment E=1.5e-34

Best Hits

KEGG orthology group: K01709, CDP-glucose 4,6-dehydratase [EC: 4.2.1.45] (inferred from 94% identity to pfo:Pfl01_1511)

MetaCyc: 54% identical to CDP-D-glucose-4,6-dehydratase monomer (Yersinia pseudotuberculosis)
CDP-glucose 4,6-dehydratase. [EC: 4.2.1.45]

Predicted SEED Role

"Similar to CDP-glucose 4,6-dehydratase (EC 4.2.1.45)" (EC 4.2.1.45)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.45

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166PEF5 at UniProt or InterPro

Protein Sequence (360 amino acids)

>Pf1N1B4_3578 Similar to CDP-glucose 4,6-dehydratase (EC 4.2.1.45) (Pseudomonas fluorescens FW300-N1B4)
MEAMGLSSQFWRGKRVLLTGHTGFKGSWLTLWLQSLGAEVSGFSLDPSTEPSLFELARVH
EGINDQRGDLRDLGALLELIAETQPEIVLHLAAQPLVREGYRDPLGTYSSNVMGTLNLLE
AIRQVGGVRACVLVTTDKVYANKEWLWPYRENEALGGHDPYSSSKACCELLAQSYAASFF
PADKYAEHGLALATARAGNVLGGGDFAPERLIPDVLKAWSADEPVTLRYPQAVRPWQHAL
EPLAGYLQLAAGLYEQGPEFAGAWNFGPSEADMCSVGEVVELLASRWPQARGLRIEPTDL
HEAGLLRLDSSRARQLLAWQPRWSLQQCLTQTLDWHLAWQNGDDMREVTLNQLNLYRGAL