Protein Info for Pf1N1B4_3573 in Pseudomonas fluorescens FW300-N1B4

Annotation: Flagellar hook-associated protein FlgK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 686 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details TIGR02492: flagellar hook-associated protein FlgK" amino acids 2 to 367 (366 residues), 240.2 bits, see alignment E=2.8e-75 PF00460: Flg_bb_rod" amino acids 4 to 33 (30 residues), 32 bits, see alignment (E = 1.9e-11) PF22638: FlgK_D1" amino acids 92 to 322 (231 residues), 253.1 bits, see alignment E=5.3e-79 PF21158: flgK_1st_1" amino acids 340 to 419 (80 residues), 27.7 bits, see alignment E=4.6e-10 PF06429: Flg_bbr_C" amino acids 647 to 684 (38 residues), 30.4 bits, see alignment 4.4e-11

Best Hits

KEGG orthology group: K02396, flagellar hook-associated protein 1 FlgK (inferred from 79% identity to pba:PSEBR_a1464)

Predicted SEED Role

"Flagellar hook-associated protein FlgK" in subsystem Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166PE03 at UniProt or InterPro

Protein Sequence (686 amino acids)

>Pf1N1B4_3573 Flagellar hook-associated protein FlgK (Pseudomonas fluorescens FW300-N1B4)
MSLLNIGMSGLAAGQSALMTTGNNIANADTAGYSRQQTVQGTKASNQFGNVYIGTGTTLA
DVRRVYNSYLDAQLQTTTSLNSDSAAYAGQISPLDKLLSDSGTGLNGALTKFFASVQNVN
AKPGDDASRQLLLSDAQALSNRFNSISSQLTQQNANVNGNLADMADQVNKLAATVAQLNQ
KISEVSKAGGQPNDLLDARNETVRQLSTFTGAQVMERDGNLDIYLGSGQPLVMGSTASKL
EVVPSKDDPSRSAIQLNRGSSTIDITSVMTGGEMGGLLRYRSTVLDPAMNELGRVALVVA
DQMNTIQAQGIDKNGDFGSNLFNSINSAAQMAQRSVATVGNSAGSGNFDVSIEDSGKLTI
NDYKVIFTSATNYTVQRLPEGTAMGAFSTLTTPPPVIDGFSMKLTGGTAAAGDTFKITPT
RNAATNIKTEMTDSKRLAIAAPLGAAIAAGGSGTLTIPASGQPTLTTKFDVYDAATTTTI
QNGLKTSTPVKIVFGAAGGTTQTYQMLDAQGNSLSTGTIVPGQNNTLSLTIPLVDASGAA
INDPGPPIVQRTVSFDMTVAGSPGNGTAINVSLSQPGSLDNRNGSTLAGLQTRQTVDTGS
ASNGISLNDAYGKLVSGVGAKAAQGKLDSAATEAILANAKGARDSLSGVDLDEETGNLVK
YQQYYTASSQIIKAAQEIFSTLINSL