Protein Info for Pf1N1B4_3545 in Pseudomonas fluorescens FW300-N1B4

Annotation: Putative two-component system sensor kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 490 transmembrane" amino acids 9 to 30 (22 residues), see Phobius details amino acids 147 to 172 (26 residues), see Phobius details PF00672: HAMP" amino acids 171 to 221 (51 residues), 30.5 bits, see alignment 7.3e-11 PF00512: HisKA" amino acids 227 to 289 (63 residues), 50.2 bits, see alignment E=4.2e-17 PF02518: HATPase_c" amino acids 335 to 438 (104 residues), 73.6 bits, see alignment E=3.6e-24

Best Hits

KEGG orthology group: None (inferred from 92% identity to pfo:Pfl01_1481)

Predicted SEED Role

"Putative two-component system sensor kinase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (490 amino acids)

>Pf1N1B4_3545 Putative two-component system sensor kinase (Pseudomonas fluorescens FW300-N1B4)
VRSLFWRILASFWLAIALVAGLSILLGHMLNQDAWILSRHPGLNTLAEQWTQTYESQGEE
AAQDILEQRKRQYHINVQVLNEAGDPVVRGTFPRRAAAFEARQNNDDRRLPWRRLTDEYT
SAKTGDTYLLIYRVPHPELDAWHRESLLWPLSALGIALVVLTLFSLLVTFSITRPLSRLR
SAVHDLGQTTYQQNSLAKLANRRDEFGVLANDFNRMGARLQSLIGSQRQLLRDVSHELRS
PLARLRIALALAERANPEEREKLWPRLTRECDRLEALISEILVLARVDADNASAEEVDLN
ALLTTLQKDAQLGSPEQNVHLEVEPQLNLKGWPTMIERAVDNLLRNAQRFNPVGQPIEMQ
ALRQGDRIVVSVRDHGPGVEAEHLNQLAEPFYRAPGQTAAGHGLGLAIARRAAERHGGSL
VLANHPEGGFIASLELPLVPGRWFNPEPCGSGLARDGGGTFNISVPDTPLSRASPLPQGN
RFLSYYRTKR