Protein Info for Pf1N1B4_3531 in Pseudomonas fluorescens FW300-N1B4

Annotation: FIG039061: hypothetical protein related to heme utilization

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 121 transmembrane" amino acids 6 to 39 (34 residues), see Phobius details amino acids 74 to 93 (20 residues), see Phobius details amino acids 96 to 113 (18 residues), see Phobius details PF04304: DUF454" amino acids 3 to 117 (115 residues), 145.5 bits, see alignment E=3.5e-47

Best Hits

Swiss-Prot: 44% identical to YBAN_ECOLI: Inner membrane protein YbaN (ybaN) from Escherichia coli (strain K12)

KEGG orthology group: K09790, hypothetical protein (inferred from 96% identity to pba:PSEBR_a1433)

Predicted SEED Role

"Hypothetical protein DUF454" in subsystem Heme, hemin uptake and utilization systems in GramPositives

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162BQ96 at UniProt or InterPro

Protein Sequence (121 amino acids)

>Pf1N1B4_3531 FIG039061: hypothetical protein related to heme utilization (Pseudomonas fluorescens FW300-N1B4)
MLRYVLLAIGWLSVALGVIGIFLPVLPTTPFLLLAAACFARSSPRFYQWLVEHPRLGPWV
RDYLSGNGIPLKGKVYAIGLMWASILLSCYLVPLPWARGFMLTSAVLVTVYILKQKTLHK
P