Protein Info for Pf1N1B4_353 in Pseudomonas fluorescens FW300-N1B4

Annotation: DNA gyrase subunit A (EC 5.99.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 889 TIGR01063: DNA gyrase, A subunit" amino acids 8 to 841 (834 residues), 1206.1 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 32 to 508 (477 residues), 551.1 bits, see alignment E=1.9e-169 PF03989: DNA_gyraseA_C" amino acids 540 to 586 (47 residues), 35.7 bits, see alignment 4.6e-13 amino acids 591 to 638 (48 residues), 40.2 bits, see alignment 1.9e-14 amino acids 646 to 689 (44 residues), 36 bits, see alignment 3.8e-13 amino acids 694 to 738 (45 residues), 49.5 bits, see alignment 2.3e-17 amino acids 745 to 789 (45 residues), 37.8 bits, see alignment 1e-13 amino acids 794 to 840 (47 residues), 48.2 bits, see alignment 5.7e-17

Best Hits

Swiss-Prot: 67% identical to GYRA_SALTY: DNA gyrase subunit A (gyrA) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02469, DNA gyrase subunit A [EC: 5.99.1.3] (inferred from 69% identity to cja:CJA_2125)

MetaCyc: 66% identical to DNA gyrase subunit A (Escherichia coli K-12 substr. MG1655)
5.99.1.3-RXN [EC: 5.6.2.2]

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.6.2.2 or 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162AQN4 at UniProt or InterPro

Protein Sequence (889 amino acids)

>Pf1N1B4_353 DNA gyrase subunit A (EC 5.99.1.3) (Pseudomonas fluorescens FW300-N1B4)
MGELAKEILPVNIEDELKQSYLDYAMSVIVGRALPDARDGLKPVHRRVLFAMSELGNDFN
KPYKKSARVVGDVIGKYHPHGDTAVYDTIVRMAQPFSLRYLLVDGQGNFGSVDGDNAAAM
RYTEVRMTKLAHELLADLHKETVDWVPNYDGTEMIPAVMPTRIPNLLVNGSSGIAVGMAT
NIPPHNLGEVIDGCLALIDNPELTVDELMQYIPGPDFPTAAIINGRAGIIEAYRTGRGRI
YMRARSMIEDIDKVGGRQQIVITELPYQLNKARLIEKIAELVKEKKLEGITELRDESDKD
GMRVVIELRRGEVPEVILNNLYAQTQLQAVFGINIVALIDGRPRILNLKDLLEAFVRHRR
EVVTRRTVFELRKARERGHILEGQAVALSNIDPVIALIKASPTPSEAKEALISTAWESSA
VVAMVERAGADSCRPETLDPQYGLRDGKYFLSPEQAQAILELRLHRLTGLEHEKLLAEYQ
EILNQIGELIRILSSATRLMEVIREELEVIRAEYGDVRRTEILDARLDLTLGDMIPEEER
VVTISHGGYAKTQPLAAYQAQRRGGKGKSATGVKDEDYIAHLLVANSHTTLLLFSSKGKV
YWLKTYEIPEASRAARGRPLVNLLPLDSDEYITTMLPVEEYTEGHFIFMATAKGTVKKTP
LESFSRQRSVGLIALELDEGDVLISAAITDGEREVMLFSDGGKVTRFKETDVRAMGRTAR
GVRGMRLPEGQKLISMLIPEEGSQILTASARGFGKRTAITEFPEYKRGGQGVIAMVSNER
NGRLVGAVQVLDGEEIMLISDQGTLVRTRVDEVSSLGRNTQGVTLIKLASDETLVGLERV
QEPSEVEGEELEGEGLEGEEGAVFDGEVVIDDVAEDQQLDAAADEEPQE