Protein Info for Pf1N1B4_3508 in Pseudomonas fluorescens FW300-N1B4

Annotation: GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 747 PF13328: HD_4" amino acids 40 to 202 (163 residues), 153 bits, see alignment E=1.5e-48 TIGR00691: RelA/SpoT family protein" amino acids 69 to 742 (674 residues), 619.5 bits, see alignment E=3.8e-190 PF04607: RelA_SpoT" amino acids 262 to 370 (109 residues), 148.9 bits, see alignment E=1.6e-47 PF02824: TGS" amino acids 416 to 475 (60 residues), 81.5 bits, see alignment 9.3e-27 PF19296: RelA_AH_RIS" amino acids 494 to 638 (145 residues), 36.6 bits, see alignment E=1.3e-12 PF13291: ACT_4" amino acids 667 to 743 (77 residues), 65.2 bits, see alignment E=1.7e-21

Best Hits

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 98% identity to pfo:Pfl01_4217)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5, 3.1.7.2

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161ZAE4 at UniProt or InterPro

Protein Sequence (747 amino acids)

>Pf1N1B4_3508 GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2) (Pseudomonas fluorescens FW300-N1B4)
MVQVRAHQPINTDGSINLEAWLDHAVSVDLALDREALKEACEFARQAEQQANAAKNLWSE
GTSSFRTGLEIAEILADLKLDQDSLVAAVLYRGVREGQIQLPAVSQRFGPVVAKLIDGVL
RMAAISASLSPRQSLVLGTQGQVENLRKMLVAMVDDVRVALIKLAERTCAIRAVKTADDE
KRNRVAREVFDIYAPLAHRLGIGHIKWELEDLSFRYLEPDQYKQIAKLLHERRLDRERFI
ADVMTQLKEELQATGVDADISGRAKHIYSIWRKMQRKGLEFSQIYDVRAVRVLVPEMRDC
YTALGIVHTLWRHIPKEFDDYIANPKENGYRSLHTAVIGPEGKVLEVQIRTHAMHEEAEL
GVCAHWKYKGTDVKSGSNHYEEKISWLRQVLEWHEELGDIGGLAEQLRVDIEPDRVYIFT
PDGHAIDLPKGATPLDFAYRVHTEIGHNCRGAKINGRIVPLNYSLQTGEQVEIITSKHGT
PSRDWLNPNLGYITTSRARAKIVHWFKLQARDQNVAAGKTLLERELNRLGLPQVDFDKLA
EKANMKTAEDMFAALGAGDLRLAQLVNLAQQLVEPERGNEQLELIPRKATGYKPGKRGDI
QIQGVGNLMTQMAGCCQPLPGDAIVGYITQGRGVSIHRQDCASVLQLAGREPERIIQVSW
GPVPVLTYPVDIIIRAYDRSGLLRDVSQVLLNERINVLAVNTRSNKEDNTALMSLTIEIP
GLDALGRLLGRISQLPNIIETRRNRTP