Protein Info for Pf1N1B4_3440 in Pseudomonas fluorescens FW300-N1B4

Updated annotation (from data): Succinylornithine transaminase (EC 2.6.1.81)
Rationale: Specifically important for utilizing L-Arginine. Automated validation from mutant phenotype: the predicted function (SUCCORNTRANSAM-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: Succinylornithine transaminase (EC 2.6.1.81)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 87% identical to ARGD1_PSESM: Acetylornithine aminotransferase 1 (argD1) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K00821, acetylornithine/N-succinyldiaminopimelate aminotransferase [EC: 2.6.1.11 2.6.1.17] (inferred from 96% identity to pfo:Pfl01_4285)

MetaCyc: 80% identical to succinylornithine transaminase subunit (Pseudomonas aeruginosa)
Succinylornithine transaminase. [EC: 2.6.1.81]; Ornithine aminotransferase. [EC: 2.6.1.81, 2.6.1.13]; Acetylornithine transaminase. [EC: 2.6.1.81, 2.6.1.13, 2.6.1.11]

Predicted SEED Role

"Succinylornithine transaminase (EC 2.6.1.81)" in subsystem Arginine and Ornithine Degradation (EC 2.6.1.81)

MetaCyc Pathways

aspartate superpathway (21/25 steps found)
L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
L-lysine biosynthesis I (9/9 steps found)
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (15/18 steps found)
superpathway of arginine and polyamine biosynthesis (14/17 steps found)
L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
L-arginine degradation II (AST pathway) (5/5 steps found)
L-arginine degradation XIII (reductive Stickland reaction) (5/5 steps found)
L-ornithine biosynthesis I (5/5 steps found)
L-citrulline biosynthesis (7/8 steps found)
L-ornithine biosynthesis II (3/3 steps found)
L-proline biosynthesis III (from L-ornithine) (3/3 steps found)
superpathway of L-citrulline metabolism (9/12 steps found)
L-arginine degradation VI (arginase 2 pathway) (3/4 steps found)
L-N^δ-acetylornithine biosynthesis (5/7 steps found)
L-arginine degradation I (arginase pathway) (2/3 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.11, 2.6.1.17

Use Curated BLAST to search for 2.6.1.11 or 2.6.1.13 or 2.6.1.17 or 2.6.1.81

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166PB01 at UniProt or InterPro

Protein Sequence (406 amino acids)

>Pf1N1B4_3440 Succinylornithine transaminase (EC 2.6.1.81) (Pseudomonas fluorescens FW300-N1B4)
MSVEHAAVERADFDQVMVPNYAPAAFIPVRGAGSRVWDQSGRELIDFAGGIAVNVLGHAH
PALVAALTEQANKLWHVSNVFTNEPALRLAHKLVDATFAERVFFCNSGAEANEAAFKLAR
RVAHDRFGTEKYEIVAALNSFHGRTLFTVNVGGQSKYSDGFGPKITGITHVPYNDLAALK
AAVSDKTCAVVLEPIQGEGGVLPAELSYLQGARELCDAHNALLVFDEVQTGMGRSGKLFA
YQHYGVTPDILTSAKSLGGGFPIAAMLTTEDLAKHLVVGTHGTTYGGNPLACAVAEAVID
VINTPEVLNGVNAKHDKFKTRLEQIGEKYGLFTEVRGLGLLLGCVLSDAWKGKAKDIFNA
AEREGLMILQAGPDVIRFAPSLVVEDADIDAGLDRFERAAAKLTQA