Protein Info for Pf1N1B4_3433 in Pseudomonas fluorescens FW300-N1B4

Updated annotation (from data): ABC transporter for L-Arginine and L-Citrulline, permease component 2
Rationale: Specific phenotypes on L-Arginine; L-Citrulline.
Original annotation: Arginine/ornithine ABC transporter, permease protein AotM

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 232 transmembrane" amino acids 15 to 42 (28 residues), see Phobius details amino acids 55 to 77 (23 residues), see Phobius details amino acids 89 to 111 (23 residues), see Phobius details amino acids 162 to 183 (22 residues), see Phobius details amino acids 195 to 216 (22 residues), see Phobius details TIGR01726: amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family" amino acids 12 to 117 (106 residues), 72.8 bits, see alignment E=1.4e-24 PF00528: BPD_transp_1" amino acids 31 to 222 (192 residues), 63.8 bits, see alignment E=9.2e-22

Best Hits

Swiss-Prot: 47% identical to OCCM_RHIRD: Octopine transport system permease protein OccM (occM) from Rhizobium radiobacter

KEGG orthology group: K10023, arginine/ornithine transport system permease protein (inferred from 95% identity to pfo:Pfl01_4290)

MetaCyc: 36% identical to L-arginine ABC transporter membrane subunit ArtM (Escherichia coli K-12 substr. MG1655)
ABC-4-RXN [EC: 7.4.2.1]

Predicted SEED Role

"Arginine/ornithine ABC transporter, permease protein AotM" in subsystem Arginine and Ornithine Degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161Z5M0 at UniProt or InterPro

Protein Sequence (232 amino acids)

>Pf1N1B4_3433 ABC transporter for L-Arginine and L-Citrulline, permease component 2 (Pseudomonas fluorescens FW300-N1B4)
MIFDYNVIWEALPLYLGGLVTTLKLLALSLFFGLLAALPLGLMRVSKQPVVNMSAWLFTY
VIRGTPMLVQLFLIYYGLAQFEAVRESFLWPWLSSATFCACLAFAINTSAYTAEIIAGSL
RATPNGEIEAAKAMGMSRIKMYKRILLPSALRRALPQYSNEVIMMLQTTSLASIVTLIDI
TGAARTVNAQFYLPFEAYITAGVFYLCLTFILVRLFKMAEHRWLGYLAPRKH