Protein Info for Pf1N1B4_340 in Pseudomonas fluorescens FW300-N1B4
Annotation: Alpha-L-Rha alpha-1,3-L-rhamnosyltransferase (EC 2.4.1.-)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 47% identical to RFBG_SHIFL: dTDP-rhamnosyl transferase RfbG (rfbG) from Shigella flexneri
KEGG orthology group: K12991, rhamnosyltransferase [EC: 2.4.1.-] (inferred from 69% identity to pfo:Pfl01_4055)MetaCyc: 51% identical to dTDP-L-Rha:6-O-acetyl-alpha-D-GlcNAc-PP-Und alpha-(1,3)-rhamnosyltransferase (Escherichia coli O49)
2.4.1.-
Predicted SEED Role
"Alpha-L-Rha alpha-1,3-L-rhamnosyltransferase (EC 2.4.1.-)" in subsystem Rhamnose containing glycans (EC 2.4.1.-)
MetaCyc Pathways
- Escherichia coli serotype O:49 O antigen biosynthesis (2/8 steps found)
KEGG Metabolic Maps
- Anthocyanin biosynthesis
- Carotenoid biosynthesis - General
- Flavone and flavonol biosynthesis
- Flavonoid biosynthesis
- Fructose and mannose metabolism
- Glycerolipid metabolism
- Glycosphingolipid biosynthesis - ganglio series
- Glycosphingolipid biosynthesis - globo series
- Glycosphingolipid biosynthesis - lacto and neolacto series
- Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
- High-mannose type N-glycan biosynthesis
- Keratan sulfate biosynthesis
- Lipopolysaccharide biosynthesis
- N-Glycan biosynthesis
- O-Glycan biosynthesis
- O-Mannosyl glycan biosynthesis
- Peptidoglycan biosynthesis
- Sphingolipid metabolism
- Zeatin biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 2.4.1.-
Use Curated BLAST to search for 2.4.1.-
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A166MKC7 at UniProt or InterPro
Protein Sequence (307 amino acids)
>Pf1N1B4_340 Alpha-L-Rha alpha-1,3-L-rhamnosyltransferase (EC 2.4.1.-) (Pseudomonas fluorescens FW300-N1B4) MPVKHPKVAVLLAAYNGMQWIEEQLASILDQSAVDVTIYISIDPSTDGTEARCAAYSAEH HQVIVLPDAGSFGGASRNFFRLIRDVDFGSFDFISFADQDDVWHKDKLQRAVDAIQTRQV DAYSSNVTAFWPDGRTHLLDKAQPQVEWDYLFEAAGPGCTYVLSKKMAESLKAAMVENWQ PLQNVTLHDWYCYAFARSHGYRWYIDPLPSMDYRQHERNQVGANKGLSPLIARYKTIHDG WWFNQVRMIARLVGRDTDPFVKTWLHLRHRQLIKLSFSAWQCRRRARDKVFFFFICWATA LIGCKAK