Protein Info for Pf1N1B4_3359 in Pseudomonas fluorescens FW300-N1B4

Annotation: Glutamine amidotransferase, class-II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 258 PF13230: GATase_4" amino acids 1 to 256 (256 residues), 361.8 bits, see alignment E=8.4e-113

Best Hits

Swiss-Prot: 61% identical to YAFJ_ECOLI: Putative glutamine amidotransferase YafJ (yafJ) from Escherichia coli (strain K12)

KEGG orthology group: K07008, glutamine amidotransferase (inferred from 97% identity to pfo:Pfl01_1401)

Predicted SEED Role

"Glutamine amidotransferase, class-II" in subsystem Ammonia assimilation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161Z9Z9 at UniProt or InterPro

Protein Sequence (258 amino acids)

>Pf1N1B4_3359 Glutamine amidotransferase, class-II (Pseudomonas fluorescens FW300-N1B4)
MCELLGMSANVPTDIVFSFTGLMQRGGRTGPHRDGWGIAFYEGRGLRLFQDPAASSESEV
ANLVQRYPIKSEVVIGHIRQANVGKVCLSNTHPFVRELWGRNWCFAHNGQLADFNPTASF
YRPVGDTDSEAAFCDLLNRVRAAFPEPVEIEALLPDLVAACAEYRSKGVFNCLLSDGDWL
FCYCSSKLAQITRRAPFGPARLKDVDVIVDFQAETTPNDVVTVIATEPLTENETWTRYEP
GQWSLWRRGECVSQGKTE