Protein Info for Pf1N1B4_3300 in Pseudomonas fluorescens FW300-N1B4

Annotation: Allophanate hydrolase (EC 3.5.1.54)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01425: Amidase
  • amino acids 27 to 440 (414 residues), 255.8 bits, E=4.6e-80
• TIGR02713: allophanate hydrolase
  • amino acids 40 to 588 (549 residues), 784.1 bits, E=3.7e-240

Best Hits

KEGG orthology group: K01457, allophanate hydrolase [EC: 3.5.1.54] (inferred from 90% identity to pfo:Pfl01_1337)

Predicted SEED Role

"Allophanate hydrolase (EC 3.5.1.54)" in subsystem Urea decomposition (EC 3.5.1.54)

MetaCyc Pathways

• L-arginine degradation V (arginine deiminase pathway) (4/4 steps found)
• L-citrulline degradation (3/3 steps found)
• cyanate degradation (3/3 steps found)
• urea degradation I (3/3 steps found)
• superpathway of allantoin degradation in yeast (5/6 steps found)
• cyanuric acid degradation II (3/5 steps found)
• cyanuric acid degradation I (2/5 steps found)
• uracil degradation III (2/5 steps found)
• superpathway of atrazine degradation (3/8 steps found)
• allantoin degradation IV (anaerobic) (2/9 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Atrazine degradation
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.54

Use Curated BLAST to search for 3.5.1.54

Sequence Analysis Tools

See A0A166P831 at UniProt or InterPro

Protein Sequence (592 amino acids)

>Pf1N1B4_3300 Allophanate hydrolase (EC 3.5.1.54) (Pseudomonas fluorescens FW300-N1B4)
MNINLQLDALRNAYHQGQITPRQLLLNLREKAAALNPDYHLFIHLLSVEELEPYLAALDG
RDPGSLPLYGVPFAIKDNIDLAGIPTTAACPAFAYVPERSATVVEQLLALGAIPLGKTNL
DQFATGLNGSRSPYGACPNSVLPEYPSGGSSAGSSLAVALGVASFSLGTDTAGSGRVPAA
LNNLVGLKASKGLISTAGVVPACRTLDCVTTFTATAREASQLLALTAHLDPRDEYSRSNP
LWNDGSAFGTPRPLRFGVPRAQDLAFFGCPEGPLLFGDAIDQLKAMGGEAVELDLSPFLE
AARLLYEGPWVAERYSVAGELMEQHPQAVLPVIRAVLDKAPAVTGVQTFRAQYRLQALKA
LCDKALEGLDCVVTPTIGRPVTRAELAAEPVLRNSELGYYTNFMNLLDYAAVAVPSGFMG
NGLPWGVTLFGRVFTDQYLLSVADTLQRQQGLAAPAPTNIARNDRARLVVCGAHLDGLAL
NWQLKQRGARLLEATHSSPDYQLYALAGGPPYRPGMVRVKEGGAAIAVEVWELPSSELGS
FLTAIPAPLGLGKVQLADGRWESGFICEPYGLEGAVDISHLGGWRAYLNDRR