Protein Info for Pf1N1B4_3246 in Pseudomonas fluorescens FW300-N1B4

Annotation: RND efflux system, inner membrane transporter CmeB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1053 transmembrane" amino acids 10 to 28 (19 residues), see Phobius details amino acids 340 to 359 (20 residues), see Phobius details amino acids 366 to 388 (23 residues), see Phobius details amino acids 395 to 414 (20 residues), see Phobius details amino acids 438 to 459 (22 residues), see Phobius details amino acids 471 to 494 (24 residues), see Phobius details amino acids 539 to 557 (19 residues), see Phobius details amino acids 877 to 895 (19 residues), see Phobius details amino acids 902 to 922 (21 residues), see Phobius details amino acids 929 to 952 (24 residues), see Phobius details amino acids 974 to 994 (21 residues), see Phobius details amino acids 1005 to 1029 (25 residues), see Phobius details PF00873: ACR_tran" amino acids 1 to 1031 (1031 residues), 1355.2 bits, see alignment E=0 TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 1 to 1048 (1048 residues), 1832.1 bits, see alignment E=0 PF03176: MMPL" amino acids 343 to 513 (171 residues), 31.9 bits, see alignment E=1.4e-11

Best Hits

Swiss-Prot: 86% identical to TTGB_PSEPK: Probable efflux pump membrane transporter TtgB (ttgB) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 65% identity to abo:ABO_0964)

MetaCyc: 66% identical to multidrug efflux pump RND permease AcrB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN0-592

Predicted SEED Role

"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162AWS9 at UniProt or InterPro

Protein Sequence (1053 amino acids)

>Pf1N1B4_3246 RND efflux system, inner membrane transporter CmeB (Pseudomonas fluorescens FW300-N1B4)
MSKFFIDRPIFAWVIALVIMLVGALSILKLPINQYPSIAPPAIAISVTYPGASAQTVQDT
VVQVIEQQLNGIDNLRYVSSESNSDGNMTITATFEQGTNSDTAQVQVQNKLNLATPLLPQ
EVQQQGIRVTKSVKNFLMVIGVVSRDGSMTKDDLSNYIVSNMQDPISRTAGVGDFQVFGA
QYAMRIWLDPAKLNNYNLTPVDVKTAIAAQNVQVSSGQLGGLPALPGQQLNATIIGKTRL
QTAEQFKAILLKVNKDGSQVRVGDVAEVGLGGENYSISAQFNGAPASGLAVKLANGANAL
DTAKALRNTIDTLKPFFPEGMEVVFPYDTTPVVTESIKGVVETLVEAIVLVFLVMFLFLQ
NFRATVITTMTVPVVLLGTFGILAAFGFSINTLTMFGMVLAIGLLVDDAIVVVENVERVM
SEEGLSPKEATKKSMGQIQGALVGIALVLSAVLLPMAFFSGSTGVIYKQFSITIVSAMAL
SVLVALIFTPALCATMLKAIPKGEHGTPKRGFFGWFNRSFDRGVKSYERGVGNILTHKAP
YLLAYLIIVVGMIWLFTRIPTAFLPEEDQGVLFAQVQTPAGSSSQRTQVVVDEMREFLLR
PSSEGGEGDAVASVFTVTGFNFAGRGQSSGMAFIMLKPWEERNADNSVFKLAARAQQHFF
TFRDAMVFAFAPPAVLELGNATGFDVFLQDRAGIGHEKLMEARNQFLGMAAQSKVLSQVR
PNGLNDEPQYQLEIDDEKASALGVTLTDINNTLSIALGSSYVNDFIDRGRVKKVYIQGLP
GARMSPEDLKKWYVRNSAGTMVPFSSFAKGEWIYGSPKLARYNGVEAMEILGAPAPGHST
GEAMAEVEALAKKLPAGVGISWTGLSYEERLSGSQAPALYALSLLMVFLCLAALYESWSI
PIAVMLVVPLGIIGALMATSMRGLSNDVYFQVGLLTTIGLAAKNAILIVEFAKELHEQGR
SLRDAAIEACRMRLRPIIMTSLAFVLGVVPLAISTGAGSGSQHAIGTGVIGGMITATVLA
IFWVPLFFVTVSSMGQRKVADQDDAIEPSKEAG