Protein Info for Pf1N1B4_3237 in Pseudomonas fluorescens FW300-N1B4
Annotation: 3-carboxy-cis,cis-muconate cycloisomerase (EC 5.5.1.2)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 78% identical to PCAB_PSEAE: 3-carboxy-cis,cis-muconate cycloisomerase (pcaB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K01857, 3-carboxy-cis,cis-muconate cycloisomerase [EC: 5.5.1.2] (inferred from 90% identity to pfo:Pfl01_1273)MetaCyc: 79% identical to subunit of 3-carboxymuconate cycloisomerase (Pseudomonas putida)
3-carboxy-cis,cis-muconate cycloisomerase. [EC: 5.5.1.2]
Predicted SEED Role
"3-carboxy-cis,cis-muconate cycloisomerase (EC 5.5.1.2)" in subsystem Protocatechuate branch of beta-ketoadipate pathway (EC 5.5.1.2)
MetaCyc Pathways
- aromatic compounds degradation via β-ketoadipate (9/9 steps found)
- protocatechuate degradation II (ortho-cleavage pathway) (4/4 steps found)
- toluene degradation III (aerobic) (via p-cresol) (7/11 steps found)
- superpathway of aromatic compound degradation via 3-oxoadipate (19/35 steps found)
- superpathway of aerobic toluene degradation (12/30 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 5.5.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A161Z557 at UniProt or InterPro
Protein Sequence (454 amino acids)
>Pf1N1B4_3237 3-carboxy-cis,cis-muconate cycloisomerase (EC 5.5.1.2) (Pseudomonas fluorescens FW300-N1B4) MNQRPGNQLFDAYFTARDMREVFCDQGRVQAMLDFEAALARAEARVGLIPQTAVASIEAA CRAEHYDFAALGEAIATAGNSAIPLVKALGKQIAKNDAEAERYVHLGATSQDVMDTGLVL QLRRALELIEADLAQLGETLATQAWRYVATPLAGRTWLQHATPVTLGMKIAGWLGAVTRS RQRLLELKPRLLVLQFGGASGTLAALGEQAMPIAEALAAELQLTLPEQPWHTQRDRLVEF GAVLGLIAGSLGKLGRDISLLMQTEAGEVFEPSAPGKGGSSTMPHKRNPVGAAVLIGAAT RVPGLLSTLFSAMPQEHERSLGLWHAEWETLPEICCLVSGSLQQALLVADGLEVEADRMT RNLDLTQGLVLAEAVSIVLAQRVGRDTAHHLLEQCCKRAVAEQRHLRAVLGDEPQVTAEL SDAELDRLLDPAHYLGQAHAWVERAVAEHFALIA