Protein Info for Pf1N1B4_3235 in Pseudomonas fluorescens FW300-N1B4
Annotation: Protocatechuate 3,4-dioxygenase beta chain (EC 1.13.11.3)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 62% identical to PCXB_BURCE: Protocatechuate 3,4-dioxygenase beta chain (pcaH) from Burkholderia cepacia
KEGG orthology group: K00449, protocatechuate 3,4-dioxygenase, beta subunit [EC: 1.13.11.3] (inferred from 94% identity to pfl:PFL_1320)MetaCyc: 58% identical to protocatechuate 3,4-dioxygenase type II beta subunit (Hydrogenophaga intermedia)
Protocatechuate 3,4-dioxygenase. [EC: 1.13.11.3]; 1.13.11.3 [EC: 1.13.11.3]
Predicted SEED Role
"Protocatechuate 3,4-dioxygenase beta chain (EC 1.13.11.3)" in subsystem Protocatechuate branch of beta-ketoadipate pathway (EC 1.13.11.3)
MetaCyc Pathways
- aromatic compounds degradation via β-ketoadipate (9/9 steps found)
- protocatechuate degradation II (ortho-cleavage pathway) (4/4 steps found)
- toluene degradation III (aerobic) (via p-cresol) (7/11 steps found)
- 4-sulfocatechol degradation (1/4 steps found)
- superpathway of aromatic compound degradation via 3-oxoadipate (19/35 steps found)
- superpathway of aerobic toluene degradation (12/30 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.13.11.3
Use Curated BLAST to search for 1.13.11.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A166P6M8 at UniProt or InterPro
Protein Sequence (234 amino acids)
>Pf1N1B4_3235 Protocatechuate 3,4-dioxygenase beta chain (EC 1.13.11.3) (Pseudomonas fluorescens FW300-N1B4) MTDKPGYRRPQAGTQPEYLHPPYQSTNLRSPSKPLVFLPHSLSEITGPTIGAERIQEQDN DLTAQHEGEPLGERIIIHGRVLDENGLPVPGILVEIWQANAAGRYNHARDLHDAPLDPNF TGTGRTVTDADGWYQFQTIKPGAYPWGNHHNAWRPAHIHFSLFGPSILTRLVTQMYFPGD PLLAYDPIYNCVPDTSAKERLIARFDLEKTIPSYALGYRWDIVLRGREATPMEK