Protein Info for Pf1N1B4_3224 in Pseudomonas fluorescens FW300-N1B4

Annotation: RNA polymerase associated protein RapA (EC 3.6.1.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 948 PF18339: Tudor_1_RapA" amino acids 5 to 55 (51 residues), 79.7 bits, see alignment 4.3e-26 PF18337: Tudor_RapA" amino acids 57 to 118 (62 residues), 77.8 bits, see alignment 2e-25 PF04851: ResIII" amino acids 156 to 315 (160 residues), 41.5 bits, see alignment E=4.6e-14 PF00270: DEAD" amino acids 172 to 315 (144 residues), 29.6 bits, see alignment E=2e-10 PF00176: SNF2-rel_dom" amino acids 172 to 345 (174 residues), 50.2 bits, see alignment E=7e-17 PF00271: Helicase_C" amino acids 474 to 584 (111 residues), 56.3 bits, see alignment E=1.3e-18 PF12137: RapA_C" amino acids 589 to 946 (358 residues), 466.2 bits, see alignment E=2.5e-143

Best Hits

Swiss-Prot: 97% identical to RAPA_PSEPF: RNA polymerase-associated protein RapA (rapA) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K03580, ATP-dependent helicase HepA [EC: 3.6.4.-] (inferred from 97% identity to pfo:Pfl01_1255)

Predicted SEED Role

"RNA polymerase associated protein RapA (EC 3.6.1.-)" (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.-

Use Curated BLAST to search for 3.6.1.- or 3.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162AWR4 at UniProt or InterPro

Protein Sequence (948 amino acids)

>Pf1N1B4_3224 RNA polymerase associated protein RapA (EC 3.6.1.-) (Pseudomonas fluorescens FW300-N1B4)
MAQQYQPGQRWISDSEAELGLGTVLAQDGRLLTVLYPATGDTRQYALRNAPLTRVRFSPG
DSITHFEGWKLTVQEVDDVDGLLVYHGLNAQNEVVTLPETQLSNFIQFRLASDRLFAGQI
DPLAWFSLRYHTLEHTSRQLQSSLWGLGGVRAQPIAHQLHIAREVADRIAPRVLLADEVG
LGKTIEAGLVIHRQLLSGRASRVLILVPENLQHQWLVEMRRRFNLQVALFDEERFIESDA
ANPFEDTQLALVALEWLVDDEKAQDALFAAGWDLLVVDEAHHLVWHEDQVSPQYSLVEQL
AEVIPGVLLLTATPEQLGQDSHFARLRLLDPNRFHDLHAFRAESENYRPVAEAVQELLDK
GRLSPEAHKTIHGFLGNEGEALLTAVNDGDTEASARLVRELLDRHGTGRVLFRNTRAAVQ
GFPERKLHPYPLPCPAEYLELPLGDHAELYPEVSFQAQPDANEEERWWKFDPRVEWLIDT
LKMLKRTKVLVICAHAETAMDLEDALRVRSGIPATVFHEGMNILERDRAAAYFADEEFGA
QVLICSEIGSEGRNFQFSHHLVLFDLPSHPDLLEQRIGRLDRIGQKHIIELHVPYLETSP
QERLYQWYHEALNAFLNTCPTGNALQHQFGPRLLPLLENADDSEWQALIDEARTERERLE
AELHTGRDRLLELNSGGAGEGDALVEDILEQDDQFALPIYMETLFDAFGIDSEDHSENAL
ILKPSEKMLDASFPLGDDEGVTITYDRNQALSREDMQFITWEHPMVQGGMDLVLSGSMGN
TAVALIKNKALKPGTVLLELLYVSEVVAPRSLQLGRYLPPAALRCLLDANGNDLSTRVSF
ETLNDQLESVPRASANKFIQAQRDQLTPRINAGEEKITPRHAERVAEAQRRLAADTEEEL
ARLTALQAVNPTVRDSELVALRKQREQGLAMLDKAALRLEAIRVLVAG