Protein Info for Pf1N1B4_321 in Pseudomonas fluorescens FW300-N1B4

Annotation: Probable short-chain dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 264 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF08659: KR" amino acids 8 to 182 (175 residues), 58.8 bits, see alignment E=1.4e-19 PF00106: adh_short" amino acids 9 to 196 (188 residues), 156.1 bits, see alignment E=1.7e-49 PF01370: Epimerase" amino acids 10 to 173 (164 residues), 28.2 bits, see alignment E=2.6e-10 PF13561: adh_short_C2" amino acids 14 to 196 (183 residues), 103.6 bits, see alignment E=2.6e-33

Best Hits

KEGG orthology group: K07124, (no description) (inferred from 85% identity to pfo:Pfl01_4040)

Predicted SEED Role

"Probable short-chain dehydrogenase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MJW6 at UniProt or InterPro

Protein Sequence (264 amino acids)

>Pf1N1B4_321 Probable short-chain dehydrogenase (Pseudomonas fluorescens FW300-N1B4)
MNSVQTQGTALVTGASSGIGAVYAERLAARGFDLLLVARDQERLEAAASKLRAAHGVQVE
VLKADLTQKDDVLKLEQRLRSDSSISLLLNNAGVAADGLLANADMDQLERLIQLNVTAVT
RLASAAAAGFSKAGRGTIINIASVVALFPEQFNATYSASKAYVLSLTQSLNAELQGTGVQ
VQAVLPGVTRTEILERSGIDASQIPADMIMEAGEMVDAALSGLDQGELITIPSLPNASEW
EAFMAARHAMAPNLSRSTAATRYK