Protein Info for Pf1N1B4_3200 in Pseudomonas fluorescens FW300-N1B4

Annotation: Outer membrane lipoprotein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 154 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF13488: Gly-zipper_Omp" amino acids 61 to 103 (43 residues), 27.9 bits, see alignment E=1.8e-10 PF05433: Rick_17kDa_Anti" amino acids 61 to 101 (41 residues), 30.9 bits, see alignment E=2e-11

Best Hits

KEGG orthology group: K06077, outer membrane lipoprotein SlyB (inferred from 93% identity to pfo:Pfl01_1231)

Predicted SEED Role

"Outer membrane lipoprotein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166P625 at UniProt or InterPro

Protein Sequence (154 amino acids)

>Pf1N1B4_3200 Outer membrane lipoprotein (Pseudomonas fluorescens FW300-N1B4)
MRKSVLLVASFSTMAMLLTGCQSSLTGDSYSRDEARRVQTIRMGTIEALRPVKIEGTKTP
IGSVAGAAVGGVGGSAIGGGRGSIVAAVIGAVAGGLIGSAAEEGLTRTQGVEITVREDDG
SMRAYVQEVQPNEVFRVGERVRIATVDGTSRVAH