Protein Info for Pf1N1B4_3195 in Pseudomonas fluorescens FW300-N1B4

Annotation: hydrolase, putative

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 840 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF17992: Agarase_CBM" amino acids 71 to 182 (112 residues), 27.3 bits, see alignment E=2.7e-10 PF02449: Glyco_hydro_42" amino acids 566 to 680 (115 residues), 22.2 bits, see alignment E=8.7e-09

Best Hits

KEGG orthology group: None (inferred from 82% identity to pfo:Pfl01_1226)

Predicted SEED Role

"hydrolase, putative"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166P605 at UniProt or InterPro

Protein Sequence (840 amino acids)

>Pf1N1B4_3195 hydrolase, putative (Pseudomonas fluorescens FW300-N1B4)
MIRRSLPAVFALMFAAPLLAAPAGQQTLFNFVRPADVVQVATQDASLPQSNAEQTAEGEV
LRRVTFNPVAQPTLRLTPQTGAWDWSQSGVMSLRIQSAMNWAVTLYVKIQSNDGKTLVSR
VDLPAGPAQTLLVPLQPSTPLSQGMKAGPPMPINFEGQRVLLASSAGELDRSQVVSVTLS
MDQPKVAQSILLERFGVQDGEAVTKAAYGGLVDAYGQSTRAKWPEKVSSDEQLKSAAAKE
QQQLKSWLAEREKQSLDKFGGWTKGPAFKASGFFRTEKRDGRWYLVTPEGHPFYSLGVNS
VTPGANQTYVAGREWMFESLPKPDEPLASYYGEGDNRGGNGADQGRAYNAGRWYDFYGAN
LQRLYGEPCASGSDTKAGVAEAAKADAAEAMTDKAAEQSAAPAATESGAGVAEAAKTGAV
EASVEKAAGQTATEPCKVVFDEKRWASHTLDRLQAWGFNTIGNWSAPVFGNADRVPYTLP
LSIVGDYASISTGTDWWGGMPDPFDPRFAMATERAVAIAARDHRDDPWLIGYFADNELAW
AGPGDDPKARYALAYGTLKMTTDVPAKRAFLKQLRDKYRNQAGLSKAWGIDLSAWELMED
PGFVPPQPSAEYPEIEADFKYFQKVFADTYFKTVSDSLKWHAPNQLLLGGRFAVSTPEAV
ESCAQYCDVLSFNMYTLQPQDGYDFARLRSLDKPVLITEFNFGSADRGPFWGGVTQLAKE
EDRGPAYANFLKQAMSEPSIVGVHWFQYLDQPVTGRLLDGENGHFGLVGITDLPFQGFVD
TVRKSNVQTVDQLGKEAEKARAEAEKATGNHEGEGGKQGAAGKGAEHGTGHAGGHSGNGH