Protein Info for Pf1N1B4_3182 in Pseudomonas fluorescens FW300-N1B4

Annotation: Penicillin acylase (EC 3.5.1.11)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 816 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details PF01804: Penicil_amidase" amino acids 52 to 802 (751 residues), 647.5 bits, see alignment E=2.1e-198

Best Hits

Swiss-Prot: 88% identical to QUIP_PSEPF: Acyl-homoserine lactone acylase QuiP (quiP) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K07116, (no description) (inferred from 88% identity to pfo:Pfl01_1213)

Predicted SEED Role

"Penicillin acylase (EC 3.5.1.11)" (EC 3.5.1.11)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.11

Use Curated BLAST to search for 3.5.1.11

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161Z9I4 at UniProt or InterPro

Protein Sequence (816 amino acids)

>Pf1N1B4_3182 Penicillin acylase (EC 3.5.1.11) (Pseudomonas fluorescens FW300-N1B4)
MASPALTHFLPRFGVAAAVAGVLSLTGCQTWNTQDTLPPTSGVQPLKGLAQNVSIRRNAM
GMPLIESNSFHDALFTLGYVHASDRISQMVTLRLLAQGRLAEMSGADLLDADRYMRAVNL
KKSAGELYKASSPRLKRFFEVYARGVNAYLFRYRDKLPADLAATGYKPEYWKPEDSALIF
CLLNFSQSANLPEEISSLMLAQTVSTDKLAWLAPSAPDEKLPVAEAEKLQGIKLNGQIPG
LSEISKATGQLSDLNLLGATSSNNWAIAPQRSRSGKSLLASDSHGPLGVPSLFSYVQIRA
PKYQASGVTIAGLPMVLGGFNGKVAWSMTTVMGDNQDLFLEKIKRQGNGLSYEVSGKWQP
AIVRNETYFVKGQKPIREAVYETRHGPLLNSAQGTALANGFGLALQTPNFTDDKTLDAFF
DLSRAQSVEKASDASREIRAIALNLVFADASNIGWQVTGRYPNRREGEGLLPSPGWEGRY
DWDGYADPMLHPYDQDPAQGWLGTANQRVIPHGYGMQLSNSWSAPERGERMAELAGVGKH
DTRSLIAMQYDQTTTFAAKLKKMFEAPGMSQPLKQAIEALPVADRGKAREAYTRLMAFDG
KLSPTSADAAIYELFLQESTKQIFLDELGPESSPAWKAFIANGKLSYAAQADHLLSREDS
PFWDDSRTPQKEDKPTILARSLAAAISAGDSQLGGDHKAWQWGKLHRYEWKNASGQTVRG
PLPAGGDHTTLNTAAFTWGQDFNTTLAPAMRFIVDFGQAEPLMGQNGSGQSGNPASAHYL
DSVEPWLKGQYISLPMQPQNFDKVYGKTRLTLTPGK