Protein Info for Pf1N1B4_3108 in Pseudomonas fluorescens FW300-N1B4

Annotation: S-(hydroxymethyl)glutathione dehydrogenase (EC 1.1.1.284)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 74% identical to FRMA_SYNY3: S-(hydroxymethyl)glutathione dehydrogenase (frmA) from Synechocystis sp. (strain PCC 6803 / Kazusa)

KEGG orthology group: K00121, S-(hydroxymethyl)glutathione dehydrogenase / alcohol dehydrogenase [EC: 1.1.1.1 1.1.1.284] (inferred from 97% identity to pba:PSEBR_a1089)

MetaCyc: 71% identical to S-(hydroxymethyl)glutathione dehydrogenase (Escherichia coli K-12 substr. MG1655)
S-(hydroxymethyl)glutathione dehydrogenase. [EC: 1.1.1.284]

Predicted SEED Role

"S-(hydroxymethyl)glutathione dehydrogenase (EC 1.1.1.284)" in subsystem Glutathione-dependent pathway of formaldehyde detoxification (EC 1.1.1.284)

MetaCyc Pathways

heterolactic fermentation (15/18 steps found)
ethanolamine utilization (5/5 steps found)
superpathway of anaerobic sucrose degradation (15/19 steps found)
3-methylbutanol biosynthesis (engineered) (6/7 steps found)
ethanol degradation II (3/3 steps found)
formaldehyde oxidation II (glutathione-dependent) (3/3 steps found)
ethanol degradation I (2/2 steps found)
mixed acid fermentation (12/16 steps found)
acetylene degradation (anaerobic) (4/5 steps found)
pyruvate fermentation to isobutanol (engineered) (4/5 steps found)
hexitol fermentation to lactate, formate, ethanol and acetate (14/19 steps found)
acetaldehyde biosynthesis I (1/1 steps found)
phytol degradation (3/4 steps found)
L-isoleucine degradation II (2/3 steps found)
L-leucine degradation III (2/3 steps found)
L-valine degradation II (2/3 steps found)
pyruvate fermentation to ethanol I (2/3 steps found)
pyruvate fermentation to ethanol III (2/3 steps found)
pyruvate fermentation to ethanol II (1/2 steps found)
protein S-nitrosylation and denitrosylation (3/5 steps found)
L-phenylalanine degradation III (2/4 steps found)
L-tyrosine degradation III (2/4 steps found)
salidroside biosynthesis (2/4 steps found)
serotonin degradation (4/7 steps found)
L-methionine degradation III (1/3 steps found)
noradrenaline and adrenaline degradation (8/13 steps found)
superpathway of fermentation (Chlamydomonas reinhardtii) (5/9 steps found)
(S)-propane-1,2-diol degradation (2/5 steps found)
phenylethanol biosynthesis (2/5 steps found)
butanol and isobutanol biosynthesis (engineered) (3/8 steps found)
superpathway of N-acetylneuraminate degradation (13/22 steps found)
superpathway of Clostridium acetobutylicum solventogenic fermentation (6/13 steps found)
superpathway of C1 compounds oxidation to CO₂ (5/12 steps found)
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (8/17 steps found)
L-tryptophan degradation V (side chain pathway) (1/13 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.1

Use Curated BLAST to search for 1.1.1.1 or 1.1.1.284

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166P4H3 at UniProt or InterPro

Protein Sequence (370 amino acids)

>Pf1N1B4_3108 S-(hydroxymethyl)glutathione dehydrogenase (EC 1.1.1.284) (Pseudomonas fluorescens FW300-N1B4)
MIKSRAAVAFEAKKPLEIVEVDVAMPKAGEVLLRVVASGVCHTDAYTLSGADPEGIFPSI
LGHEGGAVVEAIGEGVTSVAVGDHVIPLYTPECGKCKFCLSGKTNLCQAIRATQGKGLMP
DGTSRFSYKGETIFHYMGTSTFSEYTVLPEISVAKIPKEAPLEKVCLLGCGVTTGIGAVI
NTAKVKPGDTVAIFGLGGIGLSAVIGAVKAKAGRIIAIDINPAKFEIAKQLGATDCVNPK
DFDRPIQEVIVDMTDGGVDFSFECIGNVQLMRAALECCHKGWGESVIIGVAGAGQEISTR
PFQLVTGRVWRGSAFGGVRGRTELPSYVEMAQTGEIPLDTFITHTMGLEDINKAFDLMHE
GKSIRSVIHF