Protein Info for Pf1N1B4_3097 in Pseudomonas fluorescens FW300-N1B4

Annotation: Lipid-A-disaccharide synthase (EC 2.4.1.182)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 95% identical to LPXB_PSEPF: Lipid-A-disaccharide synthase (lpxB) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00748, lipid-A-disaccharide synthase [EC: 2.4.1.182] (inferred from 95% identity to pfo:Pfl01_1114)

MetaCyc: 55% identical to lipid A disaccharide synthase (Escherichia coli K-12 substr. MG1655)
Lipid-A-disaccharide synthase. [EC: 2.4.1.182]

Predicted SEED Role

"Lipid-A-disaccharide synthase (EC 2.4.1.182)" in subsystem KDO2-Lipid A biosynthesis (EC 2.4.1.182)

MetaCyc Pathways

lipid IV_A biosynthesis (P. gingivalis) (9/9 steps found)
superpathway of (Kdo)₂-lipid A biosynthesis (14/17 steps found)
lipid IV_A biosynthesis (E. coli) (6/6 steps found)
lipid IV_A biosynthesis (H. pylori) (6/6 steps found)
lipid IV_A biosynthesis (P. putida) (6/6 steps found)
lipid IV_A biosynthesis (Vibrio cholerae serogroup O1 El Tor) (6/6 steps found)
lipid IV_A biosynthesis (generic) (6/6 steps found)
lipid IV_A biosynthesis (2,3-diamino-2,3-dideoxy-D-glucopyranose-containing) (5/6 steps found)
superpathway of Kdo₂-lipid A biosynthesis (18/25 steps found)

KEGG Metabolic Maps

Lipopolysaccharide biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.182

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166P4B2 at UniProt or InterPro

Protein Sequence (375 amino acids)

>Pf1N1B4_3097 Lipid-A-disaccharide synthase (EC 2.4.1.182) (Pseudomonas fluorescens FW300-N1B4)
MANLRIALVAGEASGDILGAGLMRALKVQHPAVEFIGVGGPLMQAEGLTSYFPMERLSVM
GLVEVLGRLRELLARRKKLVADLIAEKPDVFIGIDAPDFNLNIELKLRQAGIKTVHYVSP
SVWAWRQKRVLKIREGCDLMLTLFPFEAKFYEEKGVPVRFVGHSLADAIPLEADRAAARA
ELGLPDGPLVALMPGSRGGEVGRLGALFLDTAQRVRAMRPGVRFVMPCASPQRRAQLEEL
LAGRDLPLTLLDGKSHLALAACDAVLIASGTATLEALLYKRPMVVAYRLAPLTFWILKRM
VKSPYVSLPNLLAQRLLVPELLQDDATVEALAQTLSPLIEGGQEQTRGFDEIHRTLRLDA
SNQAADAVLNLIGRA