Protein Info for Pf1N1B4_304 in Pseudomonas fluorescens FW300-N1B4

Annotation: Deoxyribonuclease TatD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 269 PF01026: TatD_DNase" amino acids 4 to 263 (260 residues), 232.5 bits, see alignment E=2.7e-73

Best Hits

Swiss-Prot: 50% identical to TATD_ENTLS: 3'-5' ssDNA/RNA exonuclease TatD (tatD) from Enterobacter lignolyticus (strain SCF1)

KEGG orthology group: K03424, TatD DNase family protein [EC: 3.1.21.-] (inferred from 93% identity to pfo:Pfl01_4021)

MetaCyc: 45% identical to 3' -> 5' ssDNA/RNA exonuclease TatD (Escherichia coli K-12 substr. MG1655)
3.1.13.-; Exodeoxyribonuclease I. [EC: 3.1.11.1]

Predicted SEED Role

"Deoxyribonuclease TatD" in subsystem Twin-arginine translocation system or YcfH

Isozymes

Compare fitness of predicted isozymes for: 3.1.11.1, 3.1.21.-

Use Curated BLAST to search for 3.1.11.1 or 3.1.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MJC8 at UniProt or InterPro

Protein Sequence (269 amino acids)

>Pf1N1B4_304 Deoxyribonuclease TatD (Pseudomonas fluorescens FW300-N1B4)
MQLIDIGVNLTNPSFADKHQAVLDRAYAAGVCQLVLTGTSVEGSEQALELCQQLDESAQR
LFATAGIHPHSASDWNADSAQRLRSLLKEPNVVAVGECGLDFNRDFSPRPQQEKVLEKHL
EMAVELQLPVFLHERDASQRLLEILRDYRDQLPAAVVHCFTGEKKALFSYLDLDLHIGIT
GWICDERRGTHLHPLVKEIKRGRLMLESDAPYLLPRSLRPKPKNGRNEPAYLTEVLREVA
LHRGESQEDLANHTTACARAFYGLPSIPQ