Protein Info for Pf1N1B4_2986 in Pseudomonas fluorescens FW300-N1B4

Annotation: sensor histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 461 transmembrane" amino acids 20 to 44 (25 residues), see Phobius details amino acids 171 to 191 (21 residues), see Phobius details PF00672: HAMP" amino acids 191 to 240 (50 residues), 33.5 bits, see alignment 6.2e-12 PF00512: HisKA" amino acids 246 to 302 (57 residues), 35.6 bits, see alignment 1.2e-12 PF02518: HATPase_c" amino acids 343 to 449 (107 residues), 79.2 bits, see alignment E=4.7e-26

Best Hits

KEGG orthology group: None (inferred from 70% identity to pfl:PFL_4996)

Predicted SEED Role

"sensor histidine kinase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166P291 at UniProt or InterPro

Protein Sequence (461 amino acids)

>Pf1N1B4_2986 sensor histidine kinase (Pseudomonas fluorescens FW300-N1B4)
MNWLKRIRARDWPQDTVARWIALTIILAMLISLALNGLFIQLAGVWARPPLTEIGLLEKA
AAITRVIEAAPAPLRNRLAQAANENGFTVTWHPDRHDLNLPAVDDPKSDIGSTILQPMLR
APDRRIEAYEPADWTEHTEDAHYALLIELSDSSWLMFSAPSRSWGLDEAPRYLIVILLVL
ISTAVVALIATRRLATPLQRFAEGARRFGVDFRAPPIEPLGPHEIRQAILAFNAMQAQLQ
HFIRDRTQMLAAISHDLRAPLTRMRLRGEFIEDSEQQQRLFRDVDEMQAMINSALEFFRD
DARLEPATQFDLAELLQTLLDDYRDQGVDITFSGPLRWVYFGRPLGLKRVITNLLDNAIK
YGSEPGIELNPSAGQVRIKVLDRGPGIPDACLEQVFVPFFRMEGSRNKSTGGVGLGLSAA
RAIVLEHGGNLTLRNRSTGGLEALVVLPVHSSSELISQHLL