Protein Info for Pf1N1B4_2974 in Pseudomonas fluorescens FW300-N1B4

Annotation: Phosphate regulon transcriptional regulatory protein PhoB (SphR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 238 PF00072: Response_reg" amino acids 7 to 117 (111 residues), 87.2 bits, see alignment E=8.5e-29 PF00486: Trans_reg_C" amino acids 155 to 231 (77 residues), 96.8 bits, see alignment E=6.1e-32

Best Hits

Swiss-Prot: 42% identical to REGX3_MYCTU: Sensory transduction protein regX3 (regX3) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

KEGG orthology group: None (inferred from 82% identity to bvi:Bcep1808_5635)

MetaCyc: 41% identical to DNA-binding transcriptional dual regulator CpxR (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Phosphate regulon transcriptional regulatory protein PhoB (SphR)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166P1Y1 at UniProt or InterPro

Protein Sequence (238 amino acids)

>Pf1N1B4_2974 Phosphate regulon transcriptional regulatory protein PhoB (SphR) (Pseudomonas fluorescens FW300-N1B4)
MEPTKRVLVVEDDLPIADLICLHLRDEQFEVVHCADGDEGMRLLQQGSWDALILDLMLPG
VDGLEICRRARAMTRYTPIIITSARSSEVHRILGLELGADDYLAKPFSMLELVARVKALL
RRVDAMARNLKMDAGSLISDGLSIDPITREVSLDGRRLDLTPREFDLLYFFVRQPGKVFS
RMDLLNAVWGYSHEGYEHTVNTHINRLRAKIEADPAQPARILTVWGRGYKFATSGEQP