Protein Info for Pf1N1B4_2967 in Pseudomonas fluorescens FW300-N1B4

Annotation: Alpha/beta hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 210 transmembrane" amino acids 103 to 122 (20 residues), see Phobius details PF00561: Abhydrolase_1" amino acids 54 to 137 (84 residues), 31.8 bits, see alignment E=2.4e-11 PF02129: Peptidase_S15" amino acids 55 to 117 (63 residues), 27.5 bits, see alignment E=5e-10 PF12146: Hydrolase_4" amino acids 56 to 139 (84 residues), 36.6 bits, see alignment E=6.4e-13 PF00975: Thioesterase" amino acids 87 to 142 (56 residues), 41.3 bits, see alignment E=4.3e-14

Best Hits

KEGG orthology group: K07018, (no description) (inferred from 91% identity to pfo:Pfl01_4700)

Predicted SEED Role

"Alpha/beta hydrolase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166P1T7 at UniProt or InterPro

Protein Sequence (210 amino acids)

>Pf1N1B4_2967 Alpha/beta hydrolase (Pseudomonas fluorescens FW300-N1B4)
MRETPVVIDGPVGQLEALYLANNEQPRGLALICHPNPVQGGTMLNKVVSTLQRTARDAGL
ITLRFNYRGVGASEGAHDMGTGEVDDAQAVAEWLRAKHPDLPLTLFGFSFGGFVAASLGG
RLEAKGEQLKHLFMVAPAVMRLGDQDQLPQQGTLTLIQPETDEVIDPQLVYDWSDALDRP
HELLKVAECGHFFHGKLTDLKDLILPRLSN