Protein Info for Pf1N1B4_2911 in Pseudomonas fluorescens FW300-N1B4
Annotation: 1-aminocyclopropane-1-carboxylate deaminase (EC 3.5.99.7)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 80% identical to 1A1D_METNO: 1-aminocyclopropane-1-carboxylate deaminase (acdS) from Methylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060)
KEGG orthology group: K01505, 1-aminocyclopropane-1-carboxylate deaminase [EC: 3.5.99.7] (inferred from 80% identity to mno:Mnod_5479)Predicted SEED Role
"1-aminocyclopropane-1-carboxylate deaminase (EC 3.5.99.7)" (EC 3.5.99.7)
MetaCyc Pathways
- superpathway of branched chain amino acid biosynthesis (17/17 steps found)
- superpathway of L-isoleucine biosynthesis I (13/13 steps found)
- L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
- L-methionine degradation II (2/3 steps found)
- L-threonine degradation I (4/6 steps found)
- superpathway of L-threonine metabolism (12/18 steps found)
- hypoglycin biosynthesis (4/14 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.5.99.7
Use Curated BLAST to search for 3.5.99.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A166P0Q7 at UniProt or InterPro
Protein Sequence (337 amino acids)
>Pf1N1B4_2911 1-aminocyclopropane-1-carboxylate deaminase (EC 3.5.99.7) (Pseudomonas fluorescens FW300-N1B4) MLETFERYPLMFGPTPIEKLNRLSACLGGNIELYAKREDCNSGLAFGGNKIRKLEYIIPD AIASQADTLVSIGGVQSNHTRQVAAVAAKLGMKCRLVQESWVPFPDAVYDRVGNILMSRV LGAEIEFVDEGFDIGIRESWERALEDVKAKGGKPYAIPAGASMHKYGGLGYVGFAEEVRE QEARMGIKFDYIIVCTVTGSTHAGMLVGFAKDGRARNVIGIDASATPEQTRAQVLAIAQH TAQLIGLGQEITADDVVLKEDYAYPAYGIPSEETNAAIRLCARMEGMMTDPVYEGKSMQG LIDLVRQGFFPTGSKVLYAHLGGVPAINAYSYIYRNG