Protein Info for Pf1N1B4_2859 in Pseudomonas fluorescens FW300-N1B4

Annotation: Two-component sensor CbrA: intrcellular carbon:nitrogen balance

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 982 transmembrane" amino acids 6 to 25 (20 residues), see Phobius details amino acids 46 to 65 (20 residues), see Phobius details amino acids 71 to 91 (21 residues), see Phobius details amino acids 112 to 134 (23 residues), see Phobius details amino acids 153 to 171 (19 residues), see Phobius details amino acids 183 to 207 (25 residues), see Phobius details amino acids 239 to 258 (20 residues), see Phobius details amino acids 272 to 296 (25 residues), see Phobius details amino acids 320 to 352 (33 residues), see Phobius details amino acids 373 to 390 (18 residues), see Phobius details amino acids 396 to 420 (25 residues), see Phobius details amino acids 427 to 449 (23 residues), see Phobius details amino acids 469 to 491 (23 residues), see Phobius details TIGR00229: PAS domain S-box protein" amino acids 631 to 746 (116 residues), 38.7 bits, see alignment E=5.1e-14 PF00989: PAS" amino acids 633 to 676 (44 residues), 27 bits, see alignment (E = 7.7e-10) PF00512: HisKA" amino acids 756 to 816 (61 residues), 42.5 bits, see alignment 1.1e-14 PF02518: HATPase_c" amino acids 863 to 973 (111 residues), 87.9 bits, see alignment E=1.3e-28

Best Hits

KEGG orthology group: K00936, [EC: 2.7.3.-] (inferred from 98% identity to pfo:Pfl01_4807)

Predicted SEED Role

"Two-component sensor CbrA: intrcellular carbon:nitrogen balance"

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.-

Use Curated BLAST to search for 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161Z8T2 at UniProt or InterPro

Protein Sequence (982 amino acids)

>Pf1N1B4_2859 Two-component sensor CbrA: intrcellular carbon:nitrogen balance (Pseudomonas fluorescens FW300-N1B4)
MSFSLTQMILISAAYLAVLFGVAWISERGMIPRAIIRHPLTYTLSLGVYASAWAFYGTVG
LAYQYGYGFLSSYLGVSGAFLLAPVLLYPILKITRTYQLSSLADLFAFRFRSTWAGALTT
IFMLIGVLPLLALQIQAVADSISILTREPVQHRVALSFCALITLFTIFFGSRHIATREKH
EGLVFAIAFESVIKLIAIGGVGLYALYGVFDGPQQLELWLLQNQTALAALHTPLQEGPWR
TLLLVFFASAIVMPHMYHMTFTENLNPRSLVSASWGLPLFLLLMSLAVPLILWAGLKLGA
TTNPEYFTLGIGIAANSKALALLAYVGGLSAASGLIIVTTLALSGMALNHLVLPLYQPPA
EGNIYRWLKWTRRALIVAIIMAGYGFYLMLGAEQDLANLGIVAFVATLQFLPGVLSVLYW
PTANRRGFIAGLLAGILVWLVTMLLPLVGNLQGFYIPLLNMIYVLDDTSWHMAAIASLAA
NVLMFTLISLFTNASPEEASAAEACAVDNVRRPQRRELHAASPQEFATQLAKPLGAKAAQ
KEVEQALRDLYLPFDERRPYALRRLRDRIEANLSGLMGPSVAQDMVETFLPYKAGGENYV
TEDIHFIESRLEDYHSRLTGLAAELDALRRYHRQTLQELPMGVCSLAKDQEILMWNKAME
ELTGIAAQRVVGSRLSTIADPWKELLQGFINLPDEHLHKQHLALDGQTRWLNLHKAAIDE
PLAPGNSGLVLLVEDLTETQMLEDKLVHSERLASIGRLAAGVAHEIGNPITGIACLAQNL
REEREDDTELTEISGQILEQTKRVSRIVQSLMSFAHAGSYQHSDEPVCLAEVAQDAIGLL
ALNRRNFEVQFYNLCDPDHWVEGDPQRLAQVLINLLSNARDASPPGSAVRVKSEAGEHTV
DLIVEDEGSGIPSSIMDRLFEPFFTTKDPGEGTGLGLALVYSIVEEHYGQITIDSPADVQ
SQRGTRIRVTLPRHVEATSAVN