Protein Info for Pf1N1B4_2856 in Pseudomonas fluorescens FW300-N1B4

Annotation: Poly(A) polymerase (EC 2.7.7.19)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 466 transmembrane" amino acids 307 to 326 (20 residues), see Phobius details TIGR01942: poly(A) polymerase" amino acids 25 to 456 (432 residues), 623.7 bits, see alignment E=7.4e-192 PF01743: PolyA_pol" amino acids 56 to 189 (134 residues), 140.8 bits, see alignment E=4.6e-45 PF12627: PolyA_pol_RNAbd" amino acids 216 to 278 (63 residues), 71 bits, see alignment E=8.7e-24 PF12626: PolyA_pol_arg_C" amino acids 332 to 448 (117 residues), 149.7 bits, see alignment E=5.6e-48

Best Hits

Swiss-Prot: 47% identical to PCNB_HAEIN: Poly(A) polymerase I (pcnB) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: None (inferred from 96% identity to pba:PSEBR_a4814)

Predicted SEED Role

"Poly(A) polymerase (EC 2.7.7.19)" in subsystem Polyadenylation bacterial (EC 2.7.7.19)

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.19

Use Curated BLAST to search for 2.7.7.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166NZT2 at UniProt or InterPro

Protein Sequence (466 amino acids)

>Pf1N1B4_2856 Poly(A) polymerase (EC 2.7.7.19) (Pseudomonas fluorescens FW300-N1B4)
MLKKLFQSFRSPKRHTQHIRSTPEVLNSGQHSLQKAQFSRYAVNIVERLQNAGYQAYLVG
GCVRDMLLGITPKDFDVATSATPEQVRAEFRNARIIGRRFKLVHIHFGREIIEVATFRAN
HPQNDEDEDSNQSSRNESGRILRDNVYGTLEEDAQRRDFTINALYYDPVSERILDYANGV
HDIRNHLIRLIGDPKQRYQEDPVRMLRAVRFAAKLNFGIEKHSATPIRELAPLLREIPSA
RLFEEVLKLFLSGYAADTFEMLVDLQLFDPLFPASAAALEYNPTYTHTLISEALINTDLR
IKQNKPVTPAFLFAALLWPALPARVLRLQERGMPPIPAMQEAAHELIAEQCQRIAIPKRF
TMPIREIWDMQERLPRRSGKRADLLLDNPRFRAGYDFLLLRESAGEQTDGLGEWWTDYQD
ANDSERRDMIRDLSGKDDGTGAPRKRRRSGGAKRKRAAGAPSATGE