Protein Info for Pf1N1B4_2805 in Pseudomonas fluorescens FW300-N1B4

Annotation: Isoleucyl-tRNA synthetase (EC 6.1.1.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 943 TIGR00392: isoleucine--tRNA ligase" amino acids 15 to 848 (834 residues), 896.1 bits, see alignment E=1.3e-273 PF00133: tRNA-synt_1" amino acids 27 to 646 (620 residues), 462.1 bits, see alignment E=3.9e-142 PF09334: tRNA-synt_1g" amino acids 58 to 198 (141 residues), 36.3 bits, see alignment E=7.5e-13 PF08264: Anticodon_1" amino acids 691 to 847 (157 residues), 92.4 bits, see alignment E=6.4e-30 PF06827: zf-FPG_IleRS" amino acids 905 to 932 (28 residues), 28.4 bits, see alignment (E = 2.8e-10)

Best Hits

Swiss-Prot: 94% identical to SYI_PSEPF: Isoleucine--tRNA ligase (ileS) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 94% identity to pfo:Pfl01_4852)

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166NYS4 at UniProt or InterPro

Protein Sequence (943 amino acids)

>Pf1N1B4_2805 Isoleucyl-tRNA synthetase (EC 6.1.1.5) (Pseudomonas fluorescens FW300-N1B4)
MTDYKATLNLPDTAFPMKAGLPQREPQILQRWDSIGLYGKLREIGKDRPKFVLHDGPPYA
NGTIHIGHALNKILKDMIIRSKTLSGFDAPYVPGWDCHGLPIEHKVEVTHGKNLGADKTR
ELCRAYATEQIEGQKSEFIRLGVLGDFANPYKTMDFKNEAGEIRALAEIVKGGFVFKGLK
PVNWCFDCGSALAEAEVEYENKKSATIDVAFPIADEAKLAAAFGLPSLGKPASIVIWTTT
PWTIPANQALNVHPEFNYALVDVGDKLLVLAEELVESCLARYSLEGSVIATTTGKALELI
NFRHPFYDRLSPVYLAEYVELGAGTGVVHSSPAYGVDDFVTCKAYGMVNDDILNPVQSNG
VYATSLEFFGGQFIWKANPAIVDKLSEVGALLHTETIEHSYMHCWRHKTPLIYRATAQWF
IGMDKEPATGETLRKRAIKAIEETKFVPAWGQARLHSMIANRPDWCISRQRNWGVPIPFF
LNKESGELHPRTVELMEIVAQRVEVEGIEAWFKMDAAELLGEEAPLYDKISDTLDVWFDS
GTTHWHVLRGSHSMGHETGPRADLYLEGSDQHRGWFHSSLLTGCAIDDHAPYRELLTHGF
TVDESGRKMSKSLGNVIAPQKVNDTLGADIMRLWVASTDYSGEMAVSEQILQRSADAYRR
IRNTARFLLSNLTGFNPATDILPAEEMLALDRWAVDRTLLLQRELQEHYGEYRFWNVYSK
IHNFCVQELGGFYLDIIKDRQYTTGANSKARRSAQTALYHISEALVRWIAPILAFTADEL
WQYLPGERNESVMLNTWYEGLTELPEGFELDRAYWERIMAVKVAVNKEKEIQRAAKAVGG
NLQAEVTLFAEDALSADLAKLSNELRFVLITSTAAVAPLVQAPADAVVTEVGGLKLKVVK
SSHAKCARCWHCREDVGVNPEHPEICGRCVDNISGAGEVRHYA