Protein Info for Pf1N1B4_2764 in Pseudomonas fluorescens FW300-N1B4

Annotation: Ferredoxin--NADP(+) reductase (EC 1.18.1.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 43% identical to FENR_AZOVI: Ferredoxin--NADP reductase (fpr) from Azotobacter vinelandii

KEGG orthology group: K00528, ferredoxin--NADP+ reductase [EC: 1.18.1.2] (inferred from 88% identity to pfl:PFL_5360)

Predicted SEED Role

"Ferredoxin--NADP(+) reductase (EC 1.18.1.2)" in subsystem Biogenesis of cytochrome c oxidases (EC 1.18.1.2)

MetaCyc Pathways

ethene biosynthesis V (engineered) (19/25 steps found)
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
oxygenic photosynthesis (11/17 steps found)
NADPH to cytochrome c oxidase via plastocyanin (thylakoid membrane) (1/4 steps found)
photosynthesis light reactions (1/4 steps found)
superpathway of photosynthetic hydrogen production (2/6 steps found)

KEGG Metabolic Maps

Photosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.18.1.2

Use Curated BLAST to search for 1.18.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166NXS1 at UniProt or InterPro

Protein Sequence (258 amino acids)

>Pf1N1B4_2764 Ferredoxin--NADP(+) reductase (EC 1.18.1.2) (Pseudomonas fluorescens FW300-N1B4)
MTASAEKFTRQTLLDVQPLTPNLFTLRTTRDSGFRFRAGQFARLGVTKADGSTVWRAYSM
VSSPYDEFLEFFSIVVPGGEFTSELSRLEVGDTLLVDRQAFGYLTLDRFVDGRDLWLLST
GTGLAPFLSILQDFEVWEKFERILLVYSVREARELAYQELIAGLAQRDYLAEYAHKLQFI
ATVTREQHPGALNGRITTLMESGELERTAGVALTAEHSRVMLCGNPQMIDDTRKLLKQRD
MHLSLSRRPGQVAVENYW