Protein Info for Pf1N1B4_2717 in Pseudomonas fluorescens FW300-N1B4

Annotation: Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00693: thiamine-phosphate diphosphorylase
  • amino acids 6 to 197 (192 residues), 202.2 bits, E=2.5e-64
• PF02581: TMP-TENI
  • amino acids 6 to 184 (179 residues), 200 bits, E=1e-63

Best Hits

Swiss-Prot: 94% identical to THIE_PSEPF: Thiamine-phosphate synthase (thiE) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00788, thiamine-phosphate pyrophosphorylase [EC: 2.5.1.3] (inferred from 94% identity to pfo:Pfl01_4946)

Predicted SEED Role

"Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3)" in subsystem Thiamin biosynthesis (EC 2.5.1.3)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• superpathway of thiamine diphosphate biosynthesis I (8/10 steps found)
• thiamine diphosphate biosynthesis I (E. coli) (2/2 steps found)
• thiamine diphosphate biosynthesis II (Bacillus) (2/2 steps found)
• thiamine diphosphate salvage II (4/5 steps found)
• thiamine diphosphate salvage V (2/3 steps found)
• thiamine diphosphate biosynthesis III (Staphylococcus) (1/3 steps found)
• thiamine diphosphate biosynthesis IV (eukaryotes) (1/3 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)
• thiamine diphosphate salvage IV (yeast) (3/7 steps found)
• thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast) (3/8 steps found)

KEGG Metabolic Maps

• Thiamine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.3

Sequence Analysis Tools

See A0A166NWV3 at UniProt or InterPro

Protein Sequence (207 amino acids)

>Pf1N1B4_2717 Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3) (Pseudomonas fluorescens FW300-N1B4)
MKLRGLYAITDSQLLAGKFLSYVEAALEGGVTLLQYRDKSSDEARRLREAEALRDLCERY
KTQLIINDDAELAARLNVGVHLGQTDGPLMPVRALLGRQAIIGSTCHAQLELAEQAAKEG
ASYVAFGRFFNSNTKPGAPACSLELLDQARSKLHLPICAIGGITLDNAAPLVAHGVDLLA
VVHGLFGAESTAEVTRRARAFNELFKS