Protein Info for Pf1N1B4_2692 in Pseudomonas fluorescens FW300-N1B4

Annotation: Penicillin-binding protein 2 (PBP-2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 631 transmembrane" amino acids 21 to 41 (21 residues), see Phobius details TIGR03423: penicillin-binding protein 2" amino acids 20 to 607 (588 residues), 780 bits, see alignment E=7.7e-239 PF03717: PBP_dimer" amino acids 63 to 235 (173 residues), 154.3 bits, see alignment E=4.8e-49 PF00905: Transpeptidase" amino acids 268 to 604 (337 residues), 249.8 bits, see alignment E=3.7e-78

Best Hits

Swiss-Prot: 44% identical to MRDA_HAEIN: Peptidoglycan D,D-transpeptidase MrdA (mrdA) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K05515, penicillin-binding protein 2 (inferred from 96% identity to pfo:Pfl01_4970)

Predicted SEED Role

"Penicillin-binding protein 2 (PBP-2)" in subsystem Peptidoglycan Biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162AVI9 at UniProt or InterPro

Protein Sequence (631 amino acids)

>Pf1N1B4_2692 Penicillin-binding protein 2 (PBP-2) (Pseudomonas fluorescens FW300-N1B4)
MPQPIRIKDHEKDARLVRGRVVFGAIAVVALIGVLIARLYFLQVIQYEYHSTLSENNRVH
VQPIPPTRGLIFDRNGVVVADNRPSFSLSMTRERSGDWQQVLDVIVEVLELTPEDRVIFE
KRMRQGRRPFEPVPILFELSEEQIARIAVNQFRLPGVEVVAQLVRHYPQGAHFAHSVGYM
GRINEKELKTLDPVSYSGTHQIGKTGIERFYEPELHGQVGYEEVETNARGRVLRVLKRTE
PISGKDIVLSLDIKLQEAAEAALGGRRGAVVALDPKTGEVLAMVSQPSFDPNLFVTGISF
KAYSELRDSIDRPLFNRVLRGLYPPGSTIKPAVAIAGLDAGVVTASTRVYDPGYYQLPNY
DHKYRNWNRTGDGYVDLDTAIMRSNDTYFYDLAHKLGIDRLSAYMSKFGIGQKVSLDMFE
ESPGLMPTREWKRATRRQAWYPGETLILGIGQGYMQSTPLQLAQATALVANKGVWNRPHL
AKTLEGAKPVDENPMPDIILRDPSDWTKVNHGMQQVMHGARGTARKASIGAQYRIAGKSG
TAQVVAIKQGEKYDRSKVQERHRDHALFVGFAPADNPQIVVSVMVENGESGSGVAAPVVR
QVMDAWLLDQDGRLKAEYASPISAEATAREE