Protein Info for Pf1N1B4_2675 in Pseudomonas fluorescens FW300-N1B4

Annotation: Nitrogen regulation protein NR(I)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 637 PF14532: Sigma54_activ_2" amino acids 336 to 504 (169 residues), 65.8 bits, see alignment E=1.3e-21 PF00158: Sigma54_activat" amino acids 337 to 499 (163 residues), 217.1 bits, see alignment E=3.1e-68 PF07728: AAA_5" amino acids 356 to 475 (120 residues), 23.9 bits, see alignment E=9.1e-09 PF02954: HTH_8" amino acids 593 to 632 (40 residues), 45.8 bits, see alignment 9.9e-16

Best Hits

KEGG orthology group: None (inferred from 90% identity to pfo:Pfl01_4988)

Predicted SEED Role

"Nitrogen regulation protein NR(I)" in subsystem Ammonia assimilation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166NVP2 at UniProt or InterPro

Protein Sequence (637 amino acids)

>Pf1N1B4_2675 Nitrogen regulation protein NR(I) (Pseudomonas fluorescens FW300-N1B4)
MHDNHLSRHAQQVLTVTQGKSHLHGPGSDPSIARSWLRCLEDYHLDPALTMAPTVLEHGR
VLESRERLQQVLQIAGNEMTSLHQQLSGAGHAVLLTDARGVILNCVTAPAERKIFERAGL
WLGADWSEACEGTNGIGTCLVERQSLTIHQDEHFRGRHTGLTCSASPVFDPHGELLAVLD
VSSARHEVSRQSQFHTMALVNLSAKMIESCYFLRYFDNQWLLRFHLQAESVGLFSEGLLA
FDGEGRICAVNQSALNLLGHIRGGLLGKPVEAFFDCSLDELLGRASANASASWPLRTRDG
RRLFAVLRGQPRSIPVPVVQSPMVAERPRLSGICLGDAALQEDFRKALRVFERDVPLLIN
GETGSGKEAFAKAVHQASQRAAKSFVALNCAAIPESLIESELFGYRGGSFTGARKEGMRG
KLQQADGGTLFLDEIGDMPLSMQTRLLRVLEDRQVVPIGGEPEPVNVRIISATHRNLLER
VQDGSFREDLYYRLNGLEVALPALRERADKSQLLDFLLAEEAGGETVLIDAPARQALLDF
AWPGNVRQLRNVLRTLAALCDGGRIGLEDLPAMIRQARPVTASAVEEPSEYPLDDAERLA
LLTVLEQQRWHMTHTAEQLGVSRNTLYRKLRKHGIAR