Protein Info for Pf1N1B4_2650 in Pseudomonas fluorescens FW300-N1B4

Annotation: Riboflavin synthase eubacterial/eukaryotic (EC 2.5.1.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00187: riboflavin synthase, alpha subunit
  • amino acids 1 to 198 (198 residues), 223.5 bits, E=7.7e-71
• PF00677: Lum_binding
  • amino acids 3 to 87 (85 residues), 74 bits, E=4.1e-25
  • amino acids 100 to 184 (85 residues), 92.1 bits, E=9.3e-31

Best Hits

Swiss-Prot: 57% identical to RISA_PHOPO: Riboflavin synthase (ribE) from Photobacterium phosphoreum

KEGG orthology group: K00793, riboflavin synthase [EC: 2.5.1.9] (inferred from 96% identity to pfo:Pfl01_5018)

Predicted SEED Role

"Riboflavin synthase eubacterial/eukaryotic (EC 2.5.1.9)" (EC 2.5.1.9)

MetaCyc Pathways

• flavin biosynthesis I (bacteria and plants) (8/9 steps found)
• flavin biosynthesis III (fungi) (7/9 steps found)
• flavin biosynthesis II (archaea) (5/10 steps found)

KEGG Metabolic Maps

• Riboflavin metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.9

Sequence Analysis Tools

See A0A162AVG1 at UniProt or InterPro

Protein Sequence (220 amino acids)

>Pf1N1B4_2650 Riboflavin synthase eubacterial/eukaryotic (EC 2.5.1.9) (Pseudomonas fluorescens FW300-N1B4)
MFTGIIESIGSIRALTPKGGDVRVHVETGKLDLSDVKLGDSIAVNGVCLTAVELPGNGFA
ADVSRETLDCTAMNDLKSGSPVNLEKALTPTTRLGGHLVSGHVDGVGEVVARTENARAVE
FRIRAPKDLAKYIAHKGSITVDGTSLTVNAVDGAEFLLTIIPHTLSETIMASYQPGRRVN
LEVDLLARYLERLLLGDKAAEPTSSNITESFLAANGYLKS