Protein Info for Pf1N1B4_265 in Pseudomonas fluorescens FW300-N1B4

Annotation: DEAD-box ATP-dependent RNA helicase CshA (EC 3.6.4.13)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 557 PF00270: DEAD" amino acids 31 to 195 (165 residues), 169.9 bits, see alignment E=8.4e-54 PF04851: ResIII" amino acids 49 to 189 (141 residues), 27 bits, see alignment E=7.8e-10 PF00271: Helicase_C" amino acids 232 to 340 (109 residues), 102 bits, see alignment E=4.6e-33 PF03880: DbpA" amino acids 484 to 552 (69 residues), 68 bits, see alignment E=1.2e-22

Best Hits

KEGG orthology group: K05592, ATP-dependent RNA helicase DeaD [EC: 3.6.4.13] (inferred from 99% identity to pfs:PFLU1726)

Predicted SEED Role

"DEAD-box ATP-dependent RNA helicase CshA (EC 3.6.4.13)" (EC 3.6.4.13)

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162AQF4 at UniProt or InterPro

Protein Sequence (557 amino acids)

>Pf1N1B4_265 DEAD-box ATP-dependent RNA helicase CshA (EC 3.6.4.13) (Pseudomonas fluorescens FW300-N1B4)
MTQETGGFAAFNLNPNILAAVIATGYEEPSAIQQQSIPIIMAGHDMIGQAQTGTGKTAAF
ALPILHRIDPAKREPQALILAPTRELALQVATAFETYAKQMPGVTVVAVYGGAPMGPQLK
AIRNGAQIVVATPGRLCDHLRRDEKVLATVNHLVLDEADEMLKLGFMDDLEVIFKALPAT
RQTVLFSATLPQSIRAIAERHLRDPQHVKIQTKTQTVTAIEQAHLLVHADQKTSAVLSLL
EVEDFDALIMFVRTKQATLDLASALEAKGYKAAALNGDIAQNQRERVIESLKDGRLDIVV
ATDVAARGLDVPRITHVFNVDMPYDPESYVHRIGRTGRAGREGRALLLVTPRERRMLQVI
ERVTGQKVAEVRLPDAQAVLDARIKKLTNSLSPLVADAESTHGDLLDRLTADIGCTPRAL
AAALLRKATNAQALTLAAIEKERPLVPNNAPRGDRPERTGDRPDRGDRERRAPVPLAEGR
ARCRTALGARDGIAAKNLLGAILNEGGLAREAIGRIQVRDSFSLVELPEDGLEKLLTKLK
DTRVAGKQLKLRRYRED