Protein Info for Pf1N1B4_2615 in Pseudomonas fluorescens FW300-N1B4

Annotation: Similarity with glutathionylspermidine synthase (EC 6.3.1.8), group 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 385 PF03738: GSP_synth" amino acids 12 to 383 (372 residues), 471.3 bits, see alignment E=1.2e-145

Best Hits

KEGG orthology group: None (inferred from 95% identity to pba:PSEBR_a5013)

Predicted SEED Role

"Similarity with glutathionylspermidine synthase (EC 6.3.1.8), group 1" in subsystem Glutathionylspermidine and Trypanothione (EC 6.3.1.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166NUH7 at UniProt or InterPro

Protein Sequence (385 amino acids)

>Pf1N1B4_2615 Similarity with glutathionylspermidine synthase (EC 6.3.1.8), group 1 (Pseudomonas fluorescens FW300-N1B4)
MKKILCAERHDWKQTAESLGFLFHTIDDEPYWDESAYYQFTLKQIENDLEDPTTEIHEMC
MDLVARVVQSEELLERLSIPAPFFDMVRTSWLEGHPHLYGRMDFSYNGSGPAKLLELNYD
TPTSLYEAAAFQWGWLEQCIERGLLPKHADQFNSIDTKLHQAFAQLQLKRPFYFASMKDS
VEDKGTTDYLRLIAEKVGIESRHIDIEDIGLTSDGRFVDLEDRWIPHLFKLHAWEFIFHE
PFGAAIAQSDTQFFEPAWKSIISNKGILPLLWEFNKSHPNLLAAHLDTDPGKAVPKGWVR
KPFFSREGANIELQTADGLIVKEDGPYTDAPFILQEFAPLPKFGDSFTLVGSWVIGDQAA
GIGVREDNSLITKDSSRFLPHLILD