Protein Info for Pf1N1B4_260 in Pseudomonas fluorescens FW300-N1B4

Annotation: Peptide methionine sulfoxide reductase MsrB (EC 1.8.4.12)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00357: methionine-R-sulfoxide reductase
  • amino acids 6 to 130 (125 residues), 190.6 bits, E=5.8e-61
• PF01641: SelR
  • amino acids 10 to 128 (119 residues), 183.5 bits, E=6.2e-59

Best Hits

Swiss-Prot: 95% identical to MSRB_PSEF5: Peptide methionine sulfoxide reductase MsrB (msrB) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K07305, peptide-methionine (R)-S-oxide reductase [EC: 1.8.4.12] (inferred from 92% identity to pfo:Pfl01_3982)

MetaCyc: 58% identical to methionine sulfoxide reductase B (Escherichia coli K-12 substr. MG1655)
L-methionine (R)-S-oxide reductase. [EC: 1.8.4.14]; Peptide-methionine (R)-S-oxide reductase. [EC: 1.8.4.14, 1.8.4.12]

Predicted SEED Role

"Peptide methionine sulfoxide reductase MsrB (EC 1.8.4.12)" (EC 1.8.4.12)

KEGG Metabolic Maps

• Methionine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.8.4.12

Use Curated BLAST to search for 1.8.4.12 or 1.8.4.14

Sequence Analysis Tools

See A0A161Z3F0 at UniProt or InterPro

Protein Sequence (131 amino acids)

>Pf1N1B4_260 Peptide methionine sulfoxide reductase MsrB (EC 1.8.4.12) (Pseudomonas fluorescens FW300-N1B4)
MEKLEKTLEEWREMLDPEQYNVCRLKGTERPFSGKYNGTKTDGVYHCICCNAPLFDSKTK
FDSGCGWPSFYAPIGDSAMVEIRDVSHGMIRTEVVCAKCDAHLGHVFPDGPPPTGLRYCI
NSVCLDLVPRE