Protein Info for Pf1N1B4_2582 in Pseudomonas fluorescens FW300-N1B4

Annotation: LSU ribosomal protein L3p (L3e)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 211 TIGR03625: 50S ribosomal protein uL3" amino acids 4 to 204 (201 residues), 300.4 bits, see alignment E=3.4e-94 PF00297: Ribosomal_L3" amino acids 47 to 182 (136 residues), 58.6 bits, see alignment E=2.8e-20

Best Hits

Swiss-Prot: 100% identical to RL3_PSESM: 50S ribosomal protein L3 (rplC) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K02906, large subunit ribosomal protein L3 (inferred from 99% identity to pfl:PFL_5582)

MetaCyc: 73% identical to 50S ribosomal subunit protein L3 (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"LSU ribosomal protein L3p (L3e)" in subsystem Ribosome LSU bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0D0PLE8 at UniProt or InterPro

Protein Sequence (211 amino acids)

>Pf1N1B4_2582 LSU ribosomal protein L3p (L3e) (Pseudomonas fluorescens FW300-N1B4)
MTIGVVGRKCGMTRIFTEEGVSIPVTVIEIEPNRVTQFKTEETDGYRAVQVTVGERRASR
VTAAQAGHFAKANVAAGRTVMEFRLEEGEYQAGDLINAEIFAAGQLVDVTGQSKGKGFQG
TIKRWNFRGQDNTHGNSVSHRVPGSIGQCQTPGRVFKGKKMSGHMGAERVTVQSLEVVRV
DAERNLLLVKGAVPGATGGNLVVRPAAKARG