Protein Info for Pf1N1B4_2531 in Pseudomonas fluorescens FW300-N1B4

Annotation: Outer membrane protein Imp, required for envelope biogenesis / Organic solvent tolerance protein precursor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 937 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details PF03968: LptD_N" amino acids 155 to 287 (133 residues), 39.5 bits, see alignment E=9.4e-14 PF19838: LptD_2" amino acids 293 to 695 (403 residues), 53.7 bits, see alignment E=2.3e-18 PF04453: LptD" amino acids 399 to 843 (445 residues), 382.1 bits, see alignment E=5.8e-118

Best Hits

Swiss-Prot: 92% identical to LPTD_PSEPF: LPS-assembly protein LptD (lptD) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K04744, LPS-assembly protein (inferred from 92% identity to pfo:Pfl01_5132)

Predicted SEED Role

"Outer membrane protein Imp, required for envelope biogenesis / Organic solvent tolerance protein precursor"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166NSV0 at UniProt or InterPro

Protein Sequence (937 amino acids)

>Pf1N1B4_2531 Outer membrane protein Imp, required for envelope biogenesis / Organic solvent tolerance protein precursor (Pseudomonas fluorescens FW300-N1B4)
MALKSPAFRKKFPLLVTGSLLALQPLATSFVVAAEQYDCSVSASGAWDCAPKAPAAALPP
RPVHDGSAVSATGEAATDTGSSADTGAKPVLVTESKGRGLKSRSEDYSHLDWVPREKLTA
AQLAETGPYCSGSYIEPIRPGMNDKTDKSDAPTFIGAKASRYKQEEQIATLAGDVVMRQG
SMQAEADEANLYQAESRGELSGNVRIRDNGALIVGDHADVQLDTGEAKVDNAEYVMHKSR
IRGNALYAKRAENAIIRLKDGTYTTCEPDSNAWQLKGNNITLNPATGFGTATNVTLRVKD
IPVLYTPYIYFPIDDRRQSGFLPPTVGTGSDTGFLLVTPYYFNLAPNYDATLYPRYMSKR
GLLMEGEFRYLTESSEGQFGAAYLNDENDDRKLQSDYEKTRYMYNWQHKGGLDSRVMTQV
DYTKISDPYYFQDLQTDQIGVKSADFVNQQGSVTYRGDSYTARLNAQAYQLATVSNITPY
DRLPQITFNGQLPYHPEGLDFDYETEIVRFDRDLESGNFVNEDGTVEPRLDNNVTGLARA
NGDRLNLKPGVSLPLNWTYGFLKPSLKYQYTQYQLDLDGTGKNQIVAQQNAAAAAGTDYI
GGEFGSNQNRGVPIASIDSGLYFDRNTQWFGKNYRQTLEPRLFYLYVPEEDQEDIPVFDT
GEYTFNYASLFRDNRFSGSDRVGDENKLSLGVTNRWIEDNGFERQRISVGQALYFKDREV
QLPGIDAKTRDDAKSSVSPYALEYEYRWNRDWRTTADYNWDPDSRSPRSGSAMFHYQPED
NPNKVINAGYRYRNDQVRYDQNTGKWSVGGGDYGTPGTPGYVKDYYKIKQHDFSVIWPIV
PQWNAISRWQYDYNRNRTLEAFGGFEYDNCCWKLRLINRYWVSYDEFSQNAPENEKGDHG
VFLQIVLKGLGGLTGAKVESFLDKGIQGYREREDQAF