Protein Info for Pf1N1B4_2529 in Pseudomonas fluorescens FW300-N1B4
Annotation: 4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 88% identical to PDXA_PSEPK: 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
KEGG orthology group: K00097, 4-hydroxythreonine-4-phosphate dehydrogenase [EC: 1.1.1.262] (inferred from 94% identity to pba:PSEBR_a5137)MetaCyc: 62% identical to 4-hydroxythreonine-4-phosphate dehydrogenase (Escherichia coli K-12 substr. MG1655)
4-hydroxythreonine-4-phosphate dehydrogenase. [EC: 1.1.1.262]
Predicted SEED Role
"4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262)" in subsystem Pyridoxin (Vitamin B6) Biosynthesis (EC 1.1.1.262)
MetaCyc Pathways
- superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (11/12 steps found)
- pyridoxal 5'-phosphate biosynthesis I (7/7 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.262
Use Curated BLAST to search for 1.1.1.262
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A161Z3H9 at UniProt or InterPro
Protein Sequence (329 amino acids)
>Pf1N1B4_2529 4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262) (Pseudomonas fluorescens FW300-N1B4) VKPKRFALTPGEPAGIGPDLCLLLASQAQPYPLIAITSRDLLIERAAQLGVVVDLLPVTP DSWPDVPAPANSLYVWDTPLNAKVIAGQLDKANAAFVLETLTRAGQGCLDGDFAGMITAP VHKGVINESGIAFSGHTEFLADLTHTTQVVMMLATRGLRVALVTTHLPLREIADAITPER LERVTRILYADLQEKFGIAQPRILVCGLNPHAGEGGHLGHEEIDIIEPTLERLRGEGMDL RGPLPADTLFTPKYLEHCDAVLAMYHDQGLPVLKYKGFGAAVNVTLGLPIIRTSVDHGTA LDLAGSGKIDTGSLQVALETAYQMAETRL