Protein Info for Pf1N1B4_2505 in Pseudomonas fluorescens FW300-N1B4

Updated annotation (from data): Lysine 2-monooxygenase (EC 1.13.12.2)
Rationale: Specifically important for utilizing L-Lysine. Automated validation from mutant phenotype: the predicted function (LYSINE-2-MONOOXYGENASE-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: Lysine 2-monooxygenase (EC 1.13.12.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 560 PF13450: NAD_binding_8" amino acids 50 to 117 (68 residues), 36.7 bits, see alignment E=6.4e-13 PF01593: Amino_oxidase" amino acids 55 to 530 (476 residues), 159.1 bits, see alignment E=3.5e-50

Best Hits

KEGG orthology group: K00466, tryptophan 2-monooxygenase [EC: 1.13.12.3] (inferred from 96% identity to pfo:Pfl01_5154)

MetaCyc: 90% identical to L-lysine monooxygenase monomer (Pseudomonas putida)
Lysine 2-monooxygenase. [EC: 1.13.12.2]

Predicted SEED Role

"Lysine 2-monooxygenase (EC 1.13.12.2)" in subsystem Lysine degradation (EC 1.13.12.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.13.12.2 or 1.13.12.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166NSA8 at UniProt or InterPro

Protein Sequence (560 amino acids)

>Pf1N1B4_2505 Lysine 2-monooxygenase (EC 1.13.12.2) (Pseudomonas fluorescens FW300-N1B4)
MNKNNRHPADGKKPVTVFGPDFPFAFDDWIEHPAGLGSIPAHNHGAEVAIVGAGIAGLVA
AYELMKLGLKPVVYEASKMGGRLRSQAFNGAEGIIAELGGMRFPVSSTAFYHYVDKLGLQ
TKPFPNPLTPASGSTVIDLEGKTHYAQKLADLPALFQEVADAWADALEAGSQFSDIQQAI
RDRDVPRLKELWNTLVPLWDDRTFYDFVATSEAFAKLSFHHREVFGQVGFGTGGWDSDFP
NSMLEIFRVVMTNCDDHQHLVVGGVEQVPLGIWRHVPERCVHWPEGTSLSSLHSGAPRSG
VKKIAHAPNGRFTVTDNYGDTREYAAVLTTCQSWLLTTQIECDESLFSQKMWMALDRTRY
MQSSKTFVMVDRPFWKDKDPETGRDLMSMTLTDRLTRGTYLFDNGDDKPGVICLSYSWMS
DALKMLPHPVEKRVKLALDALKKIYPKVDIAARIIGDPITVSWEADPHFLGAFKGALPGH
YRYNQRMYAHFMQDDMPAEQRGIFIAGDDVSWTPAWVEGAVQTSLNAVWGIMKHFGGETH
AENPGPGDVFNEIGPIALPE