Protein Info for Pf1N1B4_2499 in Pseudomonas fluorescens FW300-N1B4

Annotation: Coenzyme PQQ synthesis protein E

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 380 TIGR02109: coenzyme PQQ biosynthesis enzyme PqqE" amino acids 10 to 370 (361 residues), 614.2 bits, see alignment E=6.9e-189 PF04055: Radical_SAM" amino acids 21 to 177 (157 residues), 99.3 bits, see alignment E=4.4e-32 PF13353: Fer4_12" amino acids 24 to 118 (95 residues), 27.6 bits, see alignment E=4.8e-10 PF13186: SPASM" amino acids 248 to 313 (66 residues), 34.4 bits, see alignment E=3.4e-12 TIGR04085: radical SAM additional 4Fe4S-binding SPASM domain" amino acids 255 to 340 (86 residues), 30.1 bits, see alignment E=5.2e-11

Best Hits

Swiss-Prot: 96% identical to PQQE_PSEF5: PqqA peptide cyclase (pqqE) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K06139, pyrroloquinoline quinone biosynthesis protein E (inferred from 98% identity to pfo:Pfl01_5161)

MetaCyc: 75% identical to glutamate Cgamma--tyrosine C3 ligase (Klebsiella pneumoniae)
RXN-11176 [EC: 1.21.98.4]

Predicted SEED Role

"Coenzyme PQQ synthesis protein E" in subsystem Coenzyme PQQ synthesis or Pyrroloquinoline Quinone biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.21.98.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166NS42 at UniProt or InterPro

Protein Sequence (380 amino acids)

>Pf1N1B4_2499 Coenzyme PQQ synthesis protein E (Pseudomonas fluorescens FW300-N1B4)
VLDKLPPKPEIGLPLWLLAELTYRCPLQCPYCSNPLDFAEQGKELTTEQWIKVFREAREM
GAAQLGFSGGEPLVRQDLAELIFEARKLGFYTNLITSGIGLTEQKISDFKKAGLDHIQIS
FQASDEQVNNLLAGSKKAFAQKLEMARAVKAHGYPMVLNFVTHRHNIDKIDRIIELCIAL
EADFVELATCQFYGWAQLNRVGLLPTKEQLVRAERITNEYRAKLEAEGHPCKLIFVTPDY
YEERPKACMNGWGSIFLTVTPDGTALPCHGARQLPVQFPNVRDHSMQHIWYDSFGFNRFR
GYDWMPEPCRSCDEKEKDFGGCRCQAYMLTGDASNADPVCSKSEHHGVILKAREEAEHAT
QTIEQLAFRNERNSRLIAKS