Protein Info for Pf1N1B4_2495 in Pseudomonas fluorescens FW300-N1B4

Annotation: Acyl-CoA dehydrogenase (EC 1.3.8.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 transmembrane" amino acids 524 to 538 (15 residues), see Phobius details PF12418: AcylCoA_DH_N" amino acids 6 to 31 (26 residues), 31.3 bits, see alignment (E = 4.3e-11) PF02771: Acyl-CoA_dh_N" amino acids 42 to 158 (117 residues), 52.4 bits, see alignment E=1.7e-17 PF02770: Acyl-CoA_dh_M" amino acids 164 to 273 (110 residues), 54.9 bits, see alignment E=2e-18 PF00441: Acyl-CoA_dh_1" amino acids 283 to 451 (169 residues), 80.1 bits, see alignment E=5e-26 PF12806: Acyl-CoA_dh_C" amino acids 470 to 594 (125 residues), 134.2 bits, see alignment E=7e-43

Best Hits

KEGG orthology group: None (inferred from 94% identity to pba:PSEBR_a5173)

Predicted SEED Role

"Acyl-CoA dehydrogenase (EC 1.3.8.7)" (EC 1.3.8.7)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.7

Use Curated BLAST to search for 1.3.8.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162AV43 at UniProt or InterPro

Protein Sequence (600 amino acids)

>Pf1N1B4_2495 Acyl-CoA dehydrogenase (EC 1.3.8.7) (Pseudomonas fluorescens FW300-N1B4)
MTMPEYKAPLRDMRFLIDHVFDFHSNYAALGATDASPDMINAILEEGAKFCENVLAPLNR
SGDEEGCHFDNGVVTTPTGFKQAFAQYVEGGWHGLAADPAYGGQGLPSSLGLVISEMVGS
SNTSWGMYPGLTHGAMSAIHAHGTEEQKQTYLSKLTAGQWTGTMCLTEAHCGTDLGIIKT
RAVPQTDGSYAISGSKIFISAGEHDMSDNIIHLVLAKLPDAPAGTKGISLFIVPKYLPDA
EGEAGERNGVSCGSIEHKMGIKASATCVLNFDDAKGFLIGEPNKGLNCMFTMMNHARLGT
GMQGLCLGEASFQGAIKYANDRLQMRSLTGPKAPEKVADPIIVHPDVRRMLLTMKAFNEG
NRALTYFTAQLLDVAHLSPDETARQDAEDLLAFLTPICKAFMTDTGLEVTNLGMQVFGGH
GFIREWGMEQLVRDCRIAPIYEGTNGIQALDLLGRKVLGSQGKLLRGFTRIVHKFCAANA
EHPQLGSYVAQLNGLNQQWGELTTKVGMAAMKNPDEVGAASVDYLMYSGYIILGYLWLRM
ALVAQAQLDAGSGDADYCKGKLATSEFYFKRLLPRTAAHRAAIEAGSDCLMKLPAELFAL