Protein Info for Pf1N1B4_2493 in Pseudomonas fluorescens FW300-N1B4

Annotation: Acyl-CoA dehydrogenase (EC 1.3.8.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 601 PF12418: AcylCoA_DH_N" amino acids 3 to 35 (33 residues), 44.4 bits, see alignment (E = 3.4e-15) PF02771: Acyl-CoA_dh_N" amino acids 42 to 158 (117 residues), 42.8 bits, see alignment E=1.5e-14 PF02770: Acyl-CoA_dh_M" amino acids 163 to 272 (110 residues), 53.2 bits, see alignment E=6.9e-18 PF00441: Acyl-CoA_dh_1" amino acids 282 to 450 (169 residues), 66.5 bits, see alignment E=7.7e-22 PF12806: Acyl-CoA_dh_C" amino acids 467 to 592 (126 residues), 129.4 bits, see alignment E=2.1e-41

Best Hits

KEGG orthology group: K00257, [EC: 1.3.99.-] (inferred from 98% identity to pfo:Pfl01_5170)

Predicted SEED Role

"Acyl-CoA dehydrogenase (EC 1.3.8.7)" (EC 1.3.8.7)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.7, 1.3.99.-

Use Curated BLAST to search for 1.3.8.7 or 1.3.99.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166NS03 at UniProt or InterPro

Protein Sequence (601 amino acids)

>Pf1N1B4_2493 Acyl-CoA dehydrogenase (EC 1.3.8.7) (Pseudomonas fluorescens FW300-N1B4)
MPDYKAPLRDIRFVRDELLGYEAHYQSLPACADATPDMVDAILEEGAKFCEQVLAPLNRV
GDTEGCTWSESGVKTPTGFKEAYKQFVEGGWPSLAHDVEHGGQGLPESLGLAVSEMVGEA
NWSWGMYPGLSHGAMNTISEHGTPEQQEAYLTKLVSGEWTGTMCLTEPHCGTDLGMLRTK
AEPQADGSYKVSGTKIFISAGEHDMADNIVHIVLARLPDAPAGTKGISLFIVPKFLPNAD
GSIGARNAVTCGSLEHKMGIHGNATCVMNFDAATGYLIGPANKGLNCMFTFMNTARLGTA
LQGLAHAEIGFQGGLKYARDRLQMRSLTGPKAPDKAADPIIVHPDVRRMLLTMKAFAEGN
RAMVYFTAKQVDIVKYGVDEEEKKKADALLAFMTPIAKAFMTEVGFESANHGVQIYGGHG
FIAEWGMEQNVRDSRISMLYEGTTGIQALDLLGRKVLMTQGEALKGFTKIVHKFCQTNEG
NDAVKEFVEPLAALNKEWGELTMKVGMAAMKDREEVGAASVDYLMYSGYACLAYFWADMA
RLAAEKLAAGTTEEAFYTAKLQTARFYFQRILPRTRTHVVTMLSGANNLMDMKEEDFALG
Y