Protein Info for Pf1N1B4_2460 in Pseudomonas fluorescens FW300-N1B4

Annotation: Formyltetrahydrofolate deformylase (EC 3.5.1.10)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00655: formyltetrahydrofolate deformylase
  • amino acids 9 to 285 (277 residues), 298.7 bits, E=2.5e-93
• PF00551: Formyl_trans_N
  • amino acids 91 to 266 (176 residues), 132 bits, E=1.1e-42

Best Hits

Swiss-Prot: 51% identical to PURU_CORS1: Formyltetrahydrofolate deformylase (purU) from Corynebacterium sp. (strain P-1)

KEGG orthology group: K01433, formyltetrahydrofolate deformylase [EC: 3.5.1.10] (inferred from 96% identity to pba:PSEBR_a5210)

Predicted SEED Role

"Formyltetrahydrofolate deformylase (EC 3.5.1.10)" in subsystem One-carbon metabolism by tetrahydropterines (EC 3.5.1.10)

MetaCyc Pathways

• formaldehyde oxidation VII (THF pathway) (4/4 steps found)
• folate transformations I (9/13 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.10

Use Curated BLAST to search for 3.5.1.10

Sequence Analysis Tools

See A0A166NRC2 at UniProt or InterPro

Protein Sequence (285 amino acids)

>Pf1N1B4_2460 Formyltetrahydrofolate deformylase (EC 3.5.1.10) (Pseudomonas fluorescens FW300-N1B4)
MSRAPDTWILTADCPSVLGTVDAVTRFLFEQGCYVTEHHSFDDRLSGRFFIRVEFRQPDG
FDEQAFRAGLAERGDAFGMVFELTPPHHRPKVVIMVSKADHCLNDLLYRQRIGQLSMDVV
AVVSNHPDLKPLADWHQIPYYHFPLDPNDKPSQERQVWQVVEESGAELVILARYMQVLSP
ELCRKLDGKAINIHHSLLPGFKGAKPYHQAYNKGVKLVGATAHYINNDLDEGPIIAQGVE
AVDHSHYPEDLIAKGRDIEGLTLARAVGYHIERRVFLNANRTVVL