Protein Info for Pf1N1B4_2432 in Pseudomonas fluorescens FW300-N1B4

Annotation: Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 314 transmembrane" amino acids 6 to 26 (21 residues), see Phobius details PF01965: DJ-1_PfpI" amino acids 7 to 169 (163 residues), 52 bits, see alignment E=1.2e-17 PF12833: HTH_18" amino acids 232 to 310 (79 residues), 77.2 bits, see alignment E=1.5e-25 PF00165: HTH_AraC" amino acids 271 to 305 (35 residues), 29.4 bits, see alignment 1e-10

Best Hits

Swiss-Prot: 81% identical to CDHR_PSEAE: HTH-type transcriptional regulator CdhR (cdhR) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 96% identity to pfo:Pfl01_5229)

Predicted SEED Role

"Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166NQX1 at UniProt or InterPro

Protein Sequence (314 amino acids)

>Pf1N1B4_2432 Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain (Pseudomonas fluorescens FW300-N1B4)
MSQDFYFLLMPGFSAIGFISAIEPLRVANRFRGELYRWHVLSADGGAVLASNGMSVNADA
ALEPLKKGATLLVVAGFEPLKFSSPALEHWLRRLDNEGVTLGAIDTGSFVLAEAGLLDGH
RLTLHWEAIDAFKESYPKLSVTQELFEIDRRRISSAGGTASIDLMLDLIGQAHGPELAIQ
VSEQFVLGRIRPRKDHQRMEVATRYGISNKKLVQVIGEMEQHSEPPLSTLELAESIKVTR
RQLERLFRLHLNDTPSNFYLRLRLEKARQLLRQTNMSVLEVSIACGFESPSYFTRSYRAR
FERCPREDRRTAQA