Protein Info for Pf1N1B4_2423 in Pseudomonas fluorescens FW300-N1B4

Annotation: L-serine dehydratase (EC 4.3.1.17)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 58% identical to SDHL_STRCO: L-serine dehydratase (sdaA) from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)

KEGG orthology group: K01752, L-serine dehydratase [EC: 4.3.1.17] (inferred from 96% identity to pfo:Pfl01_5237)

Predicted SEED Role

"L-serine dehydratase (EC 4.3.1.17)" in subsystem Glycine and Serine Utilization or Pyruvate Alanine Serine Interconversions (EC 4.3.1.17)

MetaCyc Pathways

glycine betaine degradation III (7/7 steps found)
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (12/15 steps found)
D-serine degradation (3/3 steps found)
L-cysteine degradation II (3/3 steps found)
L-serine degradation (3/3 steps found)
glycine degradation (3/3 steps found)
glycine betaine degradation I (6/8 steps found)
L-tryptophan degradation II (via pyruvate) (2/3 steps found)
L-methionine biosynthesis II (4/6 steps found)
purine nucleobases degradation II (anaerobic) (16/24 steps found)
felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis (2/5 steps found)
L-mimosine degradation (4/8 steps found)
glutathione-mediated detoxification I (3/8 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.17

Use Curated BLAST to search for 4.3.1.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162BMM1 at UniProt or InterPro

Protein Sequence (458 amino acids)

>Pf1N1B4_2423 L-serine dehydratase (EC 4.3.1.17) (Pseudomonas fluorescens FW300-N1B4)
MAISVFDLFKIGIGPSSSHTVGPMRAAALFVQGLREQGLLEQVRRVEVQLYGSLSATGIG
HGSDNAVIMGLMGEWPDAIDPSQIGIRIAALRETDTLLLDGRLSVPFIWARDMRLIDENL
PFHPNAMTLVAEGDHGELHRDTYYSIGGGFVVDEAQASSGVVDLDSTVLPYDFSSAVELL
SLCKQHNLRVAELMMANEKVWRSEEEIRAGLMKLWRAMQDCVEQGLKHEGILPGGLNVRR
RAAKLHRSLQELNKPNVIGSTLSAMEWVNLFALAVNEENAAGGRMVTAPTNGAAGIIPAV
LHYFMKFSEAVTDANVVDYFLSAAAVGILCKKNASISGAEVGCQGEVGSACAMAAAGLAE
ILGATPEQLCNAAEIGLEHNLGLTCDPVGGLVQVPCIERNAIAAVKAINAAQMALRGDGQ
HFISLDRVIRTMRDTGADMHDKYKETSRGGLAVSAVEC